Overview

  • Product name

    Anti-Cyclin B2/CCNB2 antibody [X121.10]
    See all Cyclin B2/CCNB2 primary antibodies
  • Description

    Mouse monoclonal [X121.10] to Cyclin B2/CCNB2
  • Host species

    Mouse
  • Specificity

    ab10839 blots only cyclin B2/CCNB2, but cross reacts strongly with two other proteins. However, these can easily be distinguished from cyclins since they are present in interphase extracts and run well below the cyclins on SDS PAGE gels.

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Xenopus laevis
  • Immunogen

    Full length native protein (purified) corresponding to Xenopus laevis Cyclin B2/CCNB2.

  • Epitope

    The epitope for X121 lies within a region spanning the amino acids 1-112.
  • Positive control

    • Xenopus egg extract.
  • General notes

     This product was previously labelled as Cyclin B2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab10839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
IP Use at an assay dependent dilution.

Target

  • Function

    Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stage

    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Information by UniProt
  • Database links

    • Alternative names

      • ccnb2 antibody
      • CCNB2_HUMAN antibody
      • CycB2 antibody
      • Cyclin B2 antibody
      • G2 mitotic specific cyclin B2 antibody
      • G2/mitotic specific cyclin B2 antibody
      • G2/mitotic-specific cyclin-B2 antibody
      • HsT17299 antibody
      • MGC108931 antibody
      • MGC140694 antibody
      see all

    Images

    • All lanes : Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839) at 1 µg/ml

      Lane 1 : Interphase Xenopus egg extracts (cyclin B destroyed)
      Lane 2 : Mitotic (CSF) Xenopus egg extracts (cyclin B present)

      Predicted band size: 48 kDa
      Observed band size: 54 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 38 kDa (possible cross reactivity), 40 kDa (possible cross reactivity)

    References

    This product has been referenced in:

    See all 4 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab10839.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up