Key features and details
- Rabbit polyclonal to Cyclin C
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cyclin C antibody
See all Cyclin C primary antibodies
DescriptionRabbit polyclonal to Cyclin C
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to Human Cyclin C aa 233-283.
Database link: P24863
- Panc1, 293T, Jurkat, H2009 and HeLa whole cell lysate (ab150035).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionComponent of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.
Tissue specificityHighest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain.
Sequence similaritiesBelongs to the cyclin family. Cyclin C subfamily.
Contains 1 cyclin N-terminal domain.
- Information by UniProt
- ccnc antibody
- CCNC_HUMAN antibody
- CycC antibody
All lanes : Anti-Cyclin C antibody (ab85927) at 0.1 µg/ml
Lane 1 : Panc1 whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : H2009 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Cyclin C with ab85927 at 1/1000 (1µg/ml). Detection: DAB.
Detection of Human Cyclin C in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85927 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab85927 has been referenced in 3 publications.
- Lu L et al. Overexpression of miR-15b Promotes Resistance to Sunitinib in Renal Cell Carcinoma. J Cancer 10:3389-3396 (2019). PubMed: 31293642
- Xiaoli AM et al. Lipogenic SREBP-1a/c transcription factors activate expression of the iron regulator hepcidin, revealing cross-talk between lipid and iron metabolisms. J Biol Chem 294:12743-12753 (2019). PubMed: 31270208
- Fukuda A et al. Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene. PLoS One 8:e72496 (2013). PubMed: 23967313