Overview

  • Product name

  • Description

    Rabbit polyclonal to Cyclin D1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog, Rhesus monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Cyclin D1.
    Database link: P24385

  • Positive control

    • WB: HeLa, MCF7 and MDA-MB-231 whole cell extracts. IP: MCF7 whole cell extract. ICC/IF: SK-N-SH cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab226977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 34 kDa.
IP 1/100 - 1/500.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Essential for the control of the cell cycle at the G1/S (start) transition.
  • Involvement in disease

    Note=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
    Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
    Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin D subfamily.
  • Post-translational
    modifications

    Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
    Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI327039 antibody
    • B cell CLL/lymphoma 1 antibody
    • B cell leukemia 1 antibody
    • B cell lymphoma 1 protein antibody
    • B-cell lymphoma 1 protein antibody
    • BCL 1 antibody
    • BCL-1 antibody
    • BCL-1 oncogene antibody
    • BCL1 antibody
    • BCL1 oncogene antibody
    • ccnd1 antibody
    • CCND1/FSTL3 fusion gene antibody
    • CCND1/FSTL3 fusion gene, included antibody
    • CCND1/IGHG1 fusion gene antibody
    • CCND1/IGHG1 fusion gene, included antibody
    • CCND1/IGLC1 fusion gene antibody
    • CCND1/IGLC1 fusion gene, included antibody
    • CCND1/PTH fusion gene antibody
    • CCND1/PTH fusion gene, included antibody
    • CCND1_HUMAN antibody
    • cD1 antibody
    • Cyl 1 antibody
    • D11S287E antibody
    • G1/S specific cyclin D1 antibody
    • G1/S-specific cyclin-D1 antibody
    • Parathyroid adenomatosis 1 antibody
    • PRAD1 antibody
    • PRAD1 oncogene antibody
    • U21B31 antibody
    see all

Images

  • All lanes : Anti-Cyclin D1 antibody (ab226977) at 1/5000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : Cyclin D1 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 34 kDa



    12% SDS-PAGE gel.

  • All lanes : Anti-Cyclin D1 antibody (ab226977) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 34 kDa



    12% SDS-PAGE

  • 4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells labeling Cyclin D1 (green) using ab226977 at 1/500 dilution in ICC/IF.

    Counterstain: Cytoskeleton marker beta Tubulin 3/ Tuj1 antibody.

  • Cyclin D1 was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 5 µg ab226977. Western blot was performed from the immunoprecipitate using ab226977. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in MCF7 whole cell extract.

    Lane 2: ab226977 IP in MCF7 whole cell extract.

References

This product has been referenced in:

  • Song J  et al. Long noncoding RNA GAS5 inhibits angiogenesis and metastasis of colorectal cancer through the Wnt/ß-catenin signaling pathway. J Cell Biochem N/A:N/A (2019). Read more (PubMed: 30672001) »
  • Ji W  et al. Combined Androgen receptor blockade overcomes the resistance of breast cancer cells to palbociclib. Int J Biol Sci 15:522-532 (2019). Read more (PubMed: 30745839) »
See all 9 Publications for this product

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