Recombinant HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR2241] to Cyclin D1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Cyclin D1 antibody [EPR2241]
See all Cyclin D1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR2241] to Cyclin D1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7, RAW264.7 and PC12 whole cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 2.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2241 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Cyclin D1 antibody [EPR2241] - C-terminal (ab134175)
- Anti-Cyclin D1 antibody [EPR2241] - BSA and Azide free (ab156448)
- Alexa Fluor® 488 Anti-Cyclin D1 antibody [EPR2241] (ab190194)
- Alexa Fluor® 647 Anti-Cyclin D1 antibody [EPR2241] (ab190563)
- Alexa Fluor® 594 Anti-Cyclin D1 antibody [EPR2241] (ab203446)
- Alexa Fluor® 555 Anti-Cyclin D1 antibody [EPR2241] (ab203448)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
Essential for the control of the cell cycle at the G1/S (start) transition. -
Involvement in disease
Note=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus. -
Sequence similarities
Belongs to the cyclin family. Cyclin D subfamily. -
Post-translational
modificationsPhosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 595 Human
- Entrez Gene: 12443 Mouse
- Entrez Gene: 58919 Rat
- Omim: 168461 Human
- SwissProt: P24385 Human
- SwissProt: P25322 Mouse
- SwissProt: P39948 Rat
- Unigene: 523852 Human
see all -
Alternative names
- AI327039 antibody
- B cell CLL/lymphoma 1 antibody
- B cell leukemia 1 antibody
see all
Images
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All lanes : HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution
Lane 1 : MCF7 Whole Cell Lysate
Lane 2 : RAW 264.7 Whole Cell Lysate
Lane 3 : PC12 Whole cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk blocking buffer before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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All lanes : HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution + PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab190564 has been referenced in 4 publications.
- Qi CC et al. Impaired Learning and Memory Ability Induced by a Bilaterally Hippocampal Injection of Streptozotocin in Mice: Involved With the Adaptive Changes of Synaptic Plasticity. Front Aging Neurosci 13:633495 (2021). PubMed: 33732137
- Verboom L et al. OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis. Cell Rep 30:2237-2247.e6 (2020). PubMed: 32075762
- Song F et al. Propofol-induced HOXA11-AS promotes proliferation, migration and invasion, but inhibits apoptosis in hepatocellular carcinoma cells by targeting miR-4458. Int J Mol Med 46:1135-1145 (2020). PubMed: 32705160
- Cottage CT et al. Targeting p16-induced senescence prevents cigarette smoke-induced emphysema by promoting IGF1/Akt1 signaling in mice. Commun Biol 2:307 (2019). PubMed: 31428695