Product nameAnti-Cyclin D1 antibody, prediluted
See all Cyclin D1 primary antibodies
DescriptionRabbit polyclonal to Cyclin D1, prediluted
Tested applicationsSuitable for: IHC-Fr, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human Cyclin D1 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P24385
- Breast carcinoma and mantle cell lymphoma.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 0.605% Tris, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 17335794|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 34 kDa. PubMed: 20802155|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
Involvement in diseaseNote=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus.
Sequence similaritiesBelongs to the cyclin family. Cyclin D subfamily.
modificationsPhosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it.
- Information by UniProt
- AI327039 antibody
- B cell CLL/lymphoma 1 antibody
- B cell leukemia 1 antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human Mantle Cell Lymphoma, staining Cyclin D1 with ab15196.
Anti-Cyclin D1 antibody, prediluted (ab15196) at 1/25 dilution + Jurkat cell lysate
Predicted band size: 34 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
This image refers to an non-prediluted version of this product.
Immunohistochemical analysis of frozen murine uterus tissue, taken on post-natal day 7. Cyclin D1 (green) was stained using ab15196.
Sections were stained with primary antibody (as supplied) for 1 hour at room temperature before incubation with a FITC-conjugated donkey anti-rabbit secondary antibody.
This product has been referenced in:
- Shelton DN et al. The role of LEF1 in endometrial gland formation and carcinogenesis. PLoS One 7:e40312 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22792274) »
- Liu X et al. Sox17 modulates Wnt3A/beta-catenin-mediated transcriptional activation of the Lef-1 promoter. Am J Physiol Lung Cell Mol Physiol 299:L694-710 (2010). WB ; Human . Read more (PubMed: 20802155) »