Anti-Cyclin D1 (phospho T286) antibody (ab62151)
Key features and details
- Rabbit polyclonal to Cyclin D1 (phospho T286)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin D1 (phospho T286) antibody
See all Cyclin D1 primary antibodies -
Description
Rabbit polyclonal to Cyclin D1 (phospho T286) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human Cyclin D1 aa 200-300 (phospho T286).
Database link: P24385 -
General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa).
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Notes |
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WB
1/500 - 1/2000. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa). |
Target
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Function
Essential for the control of the cell cycle at the G1/S (start) transition. -
Involvement in disease
Note=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus. -
Sequence similarities
Belongs to the cyclin family. Cyclin D subfamily. -
Post-translational
modificationsPhosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 595 Human
- Entrez Gene: 12443 Mouse
- Omim: 168461 Human
- SwissProt: P24385 Human
- SwissProt: P25322 Mouse
- Unigene: 523852 Human
- Unigene: 667996 Human
- Unigene: 273049 Mouse
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Alternative names
- AI327039 antibody
- B cell CLL/lymphoma 1 antibody
- B cell leukemia 1 antibody
see all
Images
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All lanes : Anti-Cyclin D1 (phospho T286) antibody (ab62151) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells treated with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from Jurkat cells treated with EGF (200ng/ml, 30mins) with immunizing phosphopeptide
Predicted band size: 33 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab62151 has been referenced in 4 publications.
- Guo Z et al. Essential meiotic structure-specific endonuclease1 (EME1) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway. Bioengineered 12:9869-9884 (2021). PubMed: 34719326
- Chen H et al. CDK4 protein is degraded by anaphase-promoting complex/cyclosome in mitosis and reaccumulates in early G1 phase to initiate a new cell cycle in HeLa cells. J Biol Chem 292:10131-10141 (2017). PubMed: 28446612
- Sun X et al. Let-7: a regulator of the ERa signaling pathway in human breast tumors and breast cancer stem cells. Oncol Rep 29:2079-87 (2013). PubMed: 23467929
- Biggar KK & Storey KB Evidence for cell cycle suppression and microRNA regulation of cyclin D1 during anoxia exposure in turtles. Cell Cycle 11:1705-13 (2012). WB . PubMed: 22510561