Anti-Cyclin D2 antibody (ab230883)
Key features and details
- Rabbit polyclonal to Cyclin D2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin D2 antibody
See all Cyclin D2 primary antibodies -
Description
Rabbit polyclonal to Cyclin D2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig -
Immunogen
Recombinant full length protein corresponding to Human Cyclin D2 aa 1-300. (NP_001750.1).
Database link: P30279 -
Positive control
- IHC-P: Human thyroid tissue. ICC/IF: U2-OS cells. WB: NIH/3T3 and SW480 cell lysate. Mouse brain, heart, lung and spleen lysate. Rat heart lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse lung normal tissue lysate - total protein (ab29297)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- SW480 whole cell lysate (ab3957)
- Mouse brain tissue lysate - total protein (ab4022)
- NIH/3T3 whole cell lysate (ab7179)
- Mouse spleen tissue lysate - total protein (ab7937)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
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ICC/IF |
1/50 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 33 kDa.
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Notes |
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IHC-P
1/100. |
ICC/IF
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 33 kDa. |
Target
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Function
Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. -
Sequence similarities
Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain. -
Cellular localization
Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. - Information by UniProt
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Database links
- Entrez Gene: 615414 Cow
- Entrez Gene: 894 Human
- Entrez Gene: 12444 Mouse
- Entrez Gene: 397162 Pig
- Entrez Gene: 64033 Rat
- Omim: 123833 Human
- SwissProt: Q0P5D3 Cow
- SwissProt: P30279 Human
see all -
Alternative names
- CCND 2 antibody
- ccnd2 antibody
- CCND2_HUMAN antibody
see all
Images
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All lanes : Anti-Cyclin D2 antibody (ab230883) at 1/500 dilution
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : SW480 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse heart lysate
Lane 5 : Mouse lung lysate
Lane 6 : Mouse spleen lysate
Lane 7 : Rat heart lysate
Predicted band size: 33 kDa -
Formalin-fixed, paraffin-embedded human thyroid tissue stained for Cyclin D2 with ab230883 at a 1/100 dilution in immunohistochemical analysis.
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U2-OS (Human bone osteosarcoma epithelial cell line) cells stained for Cyclin D2 (green) using ab230883 at 1/50 dilution in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab230883 has been referenced in 9 publications.
- Han Z et al. DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway. Front Oncol 12:1036401 (2022). PubMed: 36408174
- Xu Q et al. All-trans retinoic acid controls differentiation, proliferation, and lipolysis in isolated subcutaneous adipocytes from peripartal Holstein cows. J Dairy Sci 104:4999-5008 (2021). PubMed: 33551168
- Lin S et al. ß-Catenin/LEF-1 transcription complex is responsible for the transcriptional activation of LINC01278. Cancer Cell Int 21:380 (2021). PubMed: 34273985
- Zhang W et al. The antagonistic effects and mechanisms of microRNA-26a action in hypertensive vascular remodelling. Br J Pharmacol 178:1037-1054 (2021). PubMed: 33305374
- Bai M et al. Discovery of a novel HDACi structure that inhibits the proliferation of ovarian cancer cells in vivo and in vitro. Int J Biol Sci 17:3493-3507 (2021). PubMed: 34512161
- Yu B et al. Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression. Mol Med Rep 24:N/A (2021). PubMed: 34664684
- Sui Y et al. Insulin-like growth factor-II overexpression accelerates parthenogenetic stem cell differentiation into cardiomyocytes and improves cardiac function after acute myocardial infarction in mice. Stem Cell Res Ther 11:86 (2020). PubMed: 32102690
- Zhu S et al. Long non-coding RNA HOXA11-AS upregulates Cyclin D2 to inhibit apoptosis and promote cell cycle progression in nephroblastoma by recruiting forkhead box P2. Am J Cancer Res 10:284-298 (2020). PubMed: 32064168
- Lu Y et al. Extracellular vesicle-enclosed miR-486-5p mediates wound healing with adipose-derived stem cells by promoting angiogenesis. J Cell Mol Med 24:9590-9604 (2020). PubMed: 32666704