Key features and details
- Rabbit polyclonal to Cyclin D2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Cyclin D2 antibody
See all Cyclin D2 primary antibodies
DescriptionRabbit polyclonal to Cyclin D2
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
Recombinant full length protein corresponding to Human Cyclin D2 aa 1-289. (NP_001750.1).
MELLCHEVDPVRRAVRDRNLLRDDRVLQNLLTIEERYLPQCSYFKCVQKD IQPYMRRMVATWMLEVCEEQKCEEEVFPLAMNYLDRFLAGVPTPKSHLQL LGAVCMFLASKLKETSPLTAEKLCIYTDNSIKPQELLEWELVVLGKLKWN LAAVTPHDFIEHILRKLPQQREKLSLIRKHAQTFIALCATDFKFAMYPPS MIATGSVGAAICGLQQDEEVSSLTCDALTELLAKITNTDVDCLKACQEQI EAVLLNSLQQYRQDQRDGSKSEDELDQASTPTDVRDIDL
Database link: P30279
- IHC-P: Human thyroid tissue. ICC/IF: U2-OS cells. WB: NIH/3T3 and SW480 cell lysate. Mouse brain, heart, lung and spleen lysate. Rat heart lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
- HeLa whole cell lysate (ab150035)
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse lung normal tissue lysate - total protein (ab29297)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- SW480 whole cell lysate (ab3957)
- Mouse brain tissue lysate - total protein (ab4022)
- NIH 3T3 whole cell lysate (ab7179)
- Mouse spleen tissue lysate - total protein (ab7937)
Our Abpromise guarantee covers the use of ab230883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 33 kDa.|
FunctionRegulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Sequence similaritiesBelongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain.
Cellular localizationNucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.
- Information by UniProt
- CCND 2 antibody
- ccnd2 antibody
- CCND2_HUMAN antibody
All lanes : Anti-Cyclin D2 antibody (ab230883) at 1/500 dilution
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : SW480 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse heart lysate
Lane 5 : Mouse lung lysate
Lane 6 : Mouse spleen lysate
Lane 7 : Rat heart lysate
Predicted band size: 33 kDa
Formalin-fixed, paraffin-embedded human thyroid tissue stained for Cyclin D2 with ab230883 at a 1/100 dilution in immunohistochemical analysis.
U2-OS (Human bone osteosarcoma epithelial cell line) cells stained for Cyclin D2 (green) using ab230883 at 1/50 dilution in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230883 has been referenced in 1 publication.
- Sui Y et al. Insulin-like growth factor-II overexpression accelerates parthenogenetic stem cell differentiation into cardiomyocytes and improves cardiac function after acute myocardial infarction in mice. Stem Cell Res Ther 11:86 (2020). PubMed: 32102690