Product nameAnti-Cyclin D2 antibody
See all Cyclin D2 primary antibodies
DescriptionRabbit polyclonal to Cyclin D2
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
Recombinant full length protein corresponding to Human Cyclin D2 aa 1-289. (NP_001750.1).
MELLCHEVDPVRRAVRDRNLLRDDRVLQNLLTIEERYLPQCSYFKCVQKD IQPYMRRMVATWMLEVCEEQKCEEEVFPLAMNYLDRFLAGVPTPKSHLQL LGAVCMFLASKLKETSPLTAEKLCIYTDNSIKPQELLEWELVVLGKLKWN LAAVTPHDFIEHILRKLPQQREKLSLIRKHAQTFIALCATDFKFAMYPPS MIATGSVGAAICGLQQDEEVSSLTCDALTELLAKITNTDVDCLKACQEQI EAVLLNSLQQYRQDQRDGSKSEDELDQASTPTDVRDIDL
Database link: P30279
- IHC-P: Human thyroid tissue. ICC/IF: U2-OS cells. WB: NIH/3T3 and SW480 cell lysate. Mouse brain, heart, lung and spleen lysate. Rat heart lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
- HeLa whole cell lysate (ab150035)
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse lung normal tissue lysate - total protein (ab29297)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- SW480 whole cell lysate (ab3957)
- Mouse brain tissue lysate - total protein (ab4022)
- NIH 3T3 whole cell lysate (ab7179)
- Mouse spleen tissue lysate - total protein (ab7937)
Our Abpromise guarantee covers the use of ab230883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 33 kDa.|
FunctionRegulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Sequence similaritiesBelongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain.
Cellular localizationNucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.
- Information by UniProt
- CCND 2 antibody
- ccnd2 antibody
- CCND2_HUMAN antibody
All lanes : Anti-Cyclin D2 antibody (ab230883) at 1/500 dilution
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : SW480 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse heart lysate
Lane 5 : Mouse lung lysate
Lane 6 : Mouse spleen lysate
Lane 7 : Rat heart lysate
Predicted band size: 33 kDa
Formalin-fixed, paraffin-embedded human thyroid tissue stained for Cyclin D2 with ab230883 at a 1/100 dilution in immunohistochemical analysis.
U2-OS (Human bone osteosarcoma epithelial cell line) cells stained for Cyclin D2 (green) using ab230883 at 1/50 dilution in ICC/IF.
ab230883 has not yet been referenced specifically in any publications.