Overview

  • Product name

    Anti-Cyclin D2 antibody - N-terminal
    See all Cyclin D2 primary antibodies
  • Description

    Rabbit polyclonal to Cyclin D2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cyclin D2 (N terminal). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: P30279

  • Positive control

    • WB: RD whole cell extract. IHC-P: Human cervical carcinoma tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab226972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin D subfamily.
    Contains 1 cyclin N-terminal domain.
  • Cellular localization

    Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCND 2 antibody
    • ccnd2 antibody
    • CCND2_HUMAN antibody
    • CyclinD2 antibody
    • G1/S specific cyclin D2 antibody
    • G1/S-specific cyclin-D2 antibody
    • KIAK0002 antibody
    • MGC102758 antibody
    • MPPH3 antibody
    see all

Images

  • Anti-Cyclin D2 antibody - N-terminal (ab226972) at 1/1000 dilution + RD whole cell extract at 30 µg

    Predicted band size: 33 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded human cervical carcinoma tissue stained for Cyclin D2 using ab226972 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cyclin D2 (green) using ab226972 at 1/500 dilution in ICC/IF. Nuclear counterstained with Hoechst 33342.

References

This product has been referenced in:

  • Yu G  et al. lncRNA TUG1 Promotes Cisplatin Resistance by Regulating CCND2 via Epigenetically Silencing miR-194-5p in Bladder Cancer. Mol Ther Nucleic Acids 16:257-271 (2019). Read more (PubMed: 30925453) »
  • He X  et al. miR-4317 suppresses non-small cell lung cancer (NSCLC) by targeting fibroblast growth factor 9 (FGF9) and cyclin D2 (CCND2). J Exp Clin Cancer Res 37:230 (2018). Read more (PubMed: 30227870) »
See all 2 Publications for this product

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