Overview

  • Product name

    Anti-Cyclin D3/CCND3 antibody
    See all Cyclin D3/CCND3 primary antibodies
  • Description

    Rabbit polyclonal to Cyclin D3/CCND3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human Cyclin D3/CCND3 aa 1-166.
    Database link: P30281

  • Positive control

    • HepG2 whole cell lysate; A431 cells.
  • General notes

     This product was previously labelled as Cyclin D3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab155682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin D subfamily.
    Contains 1 cyclin N-terminal domain.
  • Cellular localization

    Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCND 3 antibody
    • Ccnd3 antibody
    • CCND3_HUMAN antibody
    • CyclinD3 antibody
    • D3 type cyclin antibody
    • G1 S specific cyclin D3 antibody
    • G1/S specific cyclin D3 antibody
    • G1/S-specific cyclin-D3 antibody
    see all

Images

  • Anti-Cyclin D3/CCND3 antibody (ab155682) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 33 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling Cyclin D3/CCND3 with ab155682 at 1/500. Lower image shows cells co-stained with Hoechst 33342.

References

ab155682 has not yet been referenced specifically in any publications.

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