Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP207] to Cyclin D3/CCND3
- Suitable for: Flow Cyt, IHC-P, WB
- Knockout validated
- Reacts with: Human
Product nameAnti-Cyclin D3/CCND3 antibody [SP207]
See all Cyclin D3/CCND3 primary antibodies
DescriptionRabbit monoclonal [SP207] to Cyclin D3/CCND3
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide within Human Cyclin D3/CCND3 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P30281
- IHC-P: Human tonsil, skin, uterus, placenta, cervix, pancreas, kidney, reactive lymph node, cervical squamous cell carcinoma, and B-cell lymphoma tissue; WB: Wild type HAP1 whole cell lysate; Flow Cyt: K562 cells.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
This product was previously labelled as Cyclin D3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab183338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
|WB||1/100. Predicted molecular weight: 33 kDa.|
FunctionRegulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Sequence similaritiesBelongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain.
Cellular localizationNucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
- Information by UniProt
- CCND 3 antibody
- Ccnd3 antibody
- CCND3_HUMAN antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue sections labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution (3.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Empty Lane
Lane 3: CCND3 knockout HAP1 whole cell lysate (20 µg)
Lane 4: K562 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab183338 observed at 33 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab183338 was shown to recognize empty when empty knockout samples were used, along with additional cross-reactive bands. Wild-type and empty knockout samples were subjected to SDS-PAGE. Ab183338 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 100 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338) at 1/100 dilution + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 33 kDa
Flow cytometric analysis of K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells, labeling Cyclin D3/CCND3 with ab183338 diluted 1/100 (green), compared with a negative control of anti-rabbit IgG (blue).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human skin tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human uterus tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human placenta tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human cervix tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human reactive lymph node tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
ab183338 has been referenced in 1 publication.
- Feng Y et al. LncRNA NR-104098 Inhibits AML Proliferation and Induces Differentiation Through Repressing EZH2 Transcription by Interacting With E2F1. Front Cell Dev Biol 8:142 (2020). PubMed: 32296698