Recombinant Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP207] to Cyclin D3/CCND3
- Suitable for: Flow Cyt, IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Cyclin D3/CCND3 antibody [SP207]
See all Cyclin D3/CCND3 primary antibodies -
Description
Rabbit monoclonal [SP207] to Cyclin D3/CCND3 -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil, skin, uterus, placenta, cervix, pancreas, kidney, reactive lymph node, cervical squamous cell carcinoma, and B-cell lymphoma tissue; WB: Wild type HAP1 whole cell lysate; Flow Cyt: K562 cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product was previously labelled as Cyclin D3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP207 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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ICC |
Human
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IHC-P |
Human
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WB |
Human
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All applications |
Mouse
Rat
Cow
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Application | Abreviews | Notes |
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Flow Cyt |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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|
IHC-P |
1/100.
Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
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WB |
1/100. Predicted molecular weight: 33 kDa.
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Notes |
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Flow Cyt
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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IHC-P
1/100. Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
WB
1/100. Predicted molecular weight: 33 kDa. |
Target
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Function
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. -
Sequence similarities
Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain. -
Cellular localization
Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. - Information by UniProt
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Database links
- Entrez Gene: 896 Human
- Entrez Gene: 12445 Mouse
- Entrez Gene: 25193 Rat
- Omim: 123834 Human
- SwissProt: P30281 Human
- SwissProt: P30282 Mouse
- SwissProt: P48961 Rat
- Unigene: 534307 Human
see all -
Alternative names
- CCND 3 antibody
- Ccnd3 antibody
- CCND3_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue sections labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution (3.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Empty Lane
Lane 3: CCND3 knockout HAP1 whole cell lysate (20 µg)
Lane 4: K562 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab183338 observed at 33 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab183338 was shown to recognize empty when empty knockout samples were used, along with additional cross-reactive bands. Wild-type and empty knockout samples were subjected to SDS-PAGE. Ab183338 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 100 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338) at 1/100 dilution + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 33 kDa -
Flow cytometric analysis of K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells, labeling Cyclin D3/CCND3 with ab183338 diluted 1/100 (green), compared with a negative control of anti-rabbit IgG (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
Immunohistochemical analysis of paraffin-embedded human skin tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
Immunohistochemical analysis of paraffin-embedded human uterus tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
Immunohistochemical analysis of paraffin-embedded human placenta tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
Immunohistochemical analysis of paraffin-embedded human cervix tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
Immunohistochemical analysis of paraffin-embedded human kidney tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
Immunohistochemical analysis of paraffin-embedded human reactive lymph node tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab183338 has been referenced in 1 publication.
- Feng Y et al. LncRNA NR-104098 Inhibits AML Proliferation and Induces Differentiation Through Repressing EZH2 Transcription by Interacting With E2F1. Front Cell Dev Biol 8:142 (2020). PubMed: 32296698