Key features and details
- Rabbit polyclonal to Cyclin D3/CCND3 (phospho T283)
- Suitable for: ELISA, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cyclin D3/CCND3 (phospho T283) antibody
See all Cyclin D3/CCND3 primary antibodies
DescriptionRabbit polyclonal to Cyclin D3/CCND3 (phospho T283)
This antibody detects endogenous levels of Cyclin D3/CCND3 only when phosphorylated at threonine 283.
Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Cyclin D3/CCND3.
- Human lung carcinoma tissue.
This product was previously labelled as Cyclin D3
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab55322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000.|
FunctionRegulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Sequence similaritiesBelongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain.
Cellular localizationNucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
- Information by UniProt
- CCND 3 antibody
- Ccnd3 antibody
- CCND3_HUMAN antibody
Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322) + K562 cell lysate (treated with UV) blocked with phospho peptide.
This image shows human lung carcinoma tissue stained with ab55322 at 1/50 dilution. The left hand image shows untreated tissue, the right hand image shows tissue treated with the immunising phosphopeptide.
ab55322 has been referenced in 1 publication.
- Jones K et al. GSK3ß mediates muscle pathology in myotonic dystrophy. J Clin Invest 122:4461-72 (2012). WB ; Human . PubMed: 23160194