Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Cyclin D3/CCND3 (phospho T283) antibody
See all Cyclin D3/CCND3 primary antibodies -
Description
Rabbit polyclonal to Cyclin D3/CCND3 (phospho T283) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of Cyclin D3/CCND3 only when phosphorylated at threonine 283.
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Tested applications
Suitable for: ELISA, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human Cyclin D3/CCND3.
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Positive control
- Human lung carcinoma tissue.
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General notes
This product was previously labelled as Cyclin D3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab55322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/5000. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/1000. |
Target
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Function
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. -
Sequence similarities
Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain. -
Cellular localization
Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. - Information by UniProt
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Database links
- Entrez Gene: 896 Human
- Entrez Gene: 12445 Mouse
- Entrez Gene: 25193 Rat
- Omim: 123834 Human
- SwissProt: P30281 Human
- SwissProt: P30282 Mouse
- SwissProt: P48961 Rat
- Unigene: 534307 Human
see all -
Alternative names
- CCND 3 antibody
- Ccnd3 antibody
- CCND3_HUMAN antibody
see all
Images
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Western blot - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322) + K562 cell lysate (treated with UV) blocked with phospho peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)This image shows human lung carcinoma tissue stained with ab55322 at 1/50 dilution. The left hand image shows untreated tissue, the right hand image shows tissue treated with the immunising phosphopeptide.
Datasheets and documents
References
This product has been referenced in:
- Jones K et al. GSK3ß mediates muscle pathology in myotonic dystrophy. J Clin Invest 122:4461-72 (2012). WB ; Human . Read more (PubMed: 23160194) »
