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    cyclin-d3ccnd3-phospho-t283-antibody-ab55322.pdf

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Cell Biology Cell Cycle Cyclins
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Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)

  • Datasheet
Reviews (1) Submit a question References (2)

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Western blot - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)

Key features and details

  • Rabbit polyclonal to Cyclin D3/CCND3 (phospho T283)
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Recombinant Human Cyclin D3/CCND3 protein (ab158041)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Cyclin D3/CCND3 (phospho T283) antibody
    See all Cyclin D3/CCND3 primary antibodies
  • Description

    Rabbit polyclonal to Cyclin D3/CCND3 (phospho T283)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin D3/CCND3 aa 200-300 (phospho T283).
    Database link: P30281

  • Positive control

    • Human lung carcinoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cyclin D Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cyclins
    • Cyclin D Family
    • Cancer
    • Cell cycle
    • Cyclins
    • Cyclin D family

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Cyclin D3/CCND3 protein (ab158041)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab55322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
WB
1/500 - 1/1000.
Notes
IHC-P
Use at an assay dependent concentration.
WB
1/500 - 1/1000.

Target

  • Function

    Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin D subfamily.
    Contains 1 cyclin N-terminal domain.
  • Cellular localization

    Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
  • Target information above from: UniProt accession P30281 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 896 Human
    • Entrez Gene: 12445 Mouse
    • Entrez Gene: 25193 Rat
    • Omim: 123834 Human
    • SwissProt: P30281 Human
    • SwissProt: P30282 Mouse
    • SwissProt: P48961 Rat
    • Unigene: 534307 Human
    • Unigene: 27291 Mouse
    • Unigene: 472101 Mouse
    • Unigene: 3483 Rat
    • Unigene: 54319 Rat
    see all
  • Alternative names

    • CCND 3 antibody
    • Ccnd3 antibody
    • CCND3_HUMAN antibody
    • CyclinD3 antibody
    • D3 type cyclin antibody
    • G1 S specific cyclin D3 antibody
    • G1/S specific cyclin D3 antibody
    • G1/S-specific cyclin-D3 antibody
    see all

Images

  • Western blot - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)
    Western blot - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)
    Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322) + K562 cell lysate (treated with UV) blocked with phospho peptide.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)
    This image shows human lung carcinoma tissue stained with ab55322 at 1/50 dilution. The left hand image shows untreated tissue, the right hand image shows tissue treated with the immunising phosphopeptide.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab55322? Please let us know so that we can cite the reference in this datasheet.

ab55322 has been referenced in 2 publications.

  • Fruit C  et al. Biological Characterization of 8-Cyclopropyl-2-(pyridin-3-yl)thiazolo[5,4-f]quinazolin-9(8H)-one, a Promising Inhibitor of DYRK1A. Pharmaceuticals (Basel) 12:N/A (2019). PubMed: 31861110
  • Jones K  et al. GSK3ß mediates muscle pathology in myotonic dystrophy. J Clin Invest 122:4461-72 (2012). WB ; Human . PubMed: 23160194

Customer reviews and Q&As

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Western blot abreview for Anti-Cyclin D3/CCND3 (phospho T283) antibody

Inconclusive
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (pre-B cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
pre-B cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Nov 20 2013

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