Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)

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Western blot - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)

Key features and details

  • Rabbit polyclonal to Cyclin D3/CCND3 (phospho T283)
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Cyclin D3/CCND3 (phospho T283) antibody
    See all Cyclin D3/CCND3 primary antibodies

  • Description

    Rabbit polyclonal to Cyclin D3/CCND3 (phospho T283)

  • Host species

    Rabbit

  • Specificity

    This antibody detects endogenous levels of Cyclin D3/CCND3 only when phosphorylated at threonine 283.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Cyclin D3/CCND3.

  • Positive control

    • Human lung carcinoma tissue.

  • General notes

     This product was previously labelled as Cyclin D3

     

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab55322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
WB
1/500 - 1/1000.
Notes
IHC-P
Use at an assay dependent concentration.
WB
1/500 - 1/1000.

Target

  • Function

    Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

  • Sequence similarities

    Belongs to the cyclin family. Cyclin D subfamily.
    Contains 1 cyclin N-terminal domain.

  • Cellular localization

    Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CCND 3 antibody
    • Ccnd3 antibody
    • CCND3_HUMAN antibody
    • CyclinD3 antibody
    • D3 type cyclin antibody
    • G1 S specific cyclin D3 antibody
    • G1/S specific cyclin D3 antibody
    • G1/S-specific cyclin-D3 antibody
    see all

Images

  • Western blot - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)
    Western blot - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)
    Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322) + K562 cell lysate (treated with UV) blocked with phospho peptide.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)
    This image shows human lung carcinoma tissue stained with ab55322 at 1/50 dilution. The left hand image shows untreated tissue, the right hand image shows tissue treated with the immunising phosphopeptide.

References (2)

Publishing research using ab55322? Please let us know so that we can cite the reference in this datasheet.

ab55322 has been referenced in 2 publications.

  • Fruit C  et al. Biological Characterization of 8-Cyclopropyl-2-(pyridin-3-yl)thiazolo[5,4-f]quinazolin-9(8H)-one, a Promising Inhibitor of DYRK1A. Pharmaceuticals (Basel) 12:N/A (2019). PubMed: 31861110
  • Jones K  et al. GSK3ß mediates muscle pathology in myotonic dystrophy. J Clin Invest 122:4461-72 (2012). WB ; Human . PubMed: 23160194

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Western blot abreview for Anti-Cyclin D3/CCND3 (phospho T283) antibody

 Inconclusive
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (pre-B cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
pre-B cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 20 2013

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