Anti-Cyclin E1 antibody [5F8C5] (ab211342)
Key features and details
- Mouse monoclonal [5F8C5] to Cyclin E1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Cyclin E1 antibody [5F8C5]
See all Cyclin E1 primary antibodies -
Description
Mouse monoclonal [5F8C5] to Cyclin E1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Cyclin E1 aa 300 to the C-terminus.
Database link: P24864 -
Positive control
- Cyclin E1 (AA: 307-410) recombinant protein; Cyclin E1 (AA: 307-410)-hIgGFc transfected HEK293 cell lysate; HeLa, K562, NIH3T3, C6, MCF7, Jurkat and A431 cell lysates; K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5F8C5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A-431 whole cell lysate (ab30132)
- NIH/3T3 whole cell lysate (ab7179)
- Jurkat whole cell lysate (ab7899)
- A-431 whole cell lysate (ab7909)
- K-562 whole cell lysate (ab7911)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 47 kDa.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 47 kDa. |
Flow Cyt
1/200 - 1/400. |
Target
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Function
Essential for the control of the cell cycle at the G1/S (start) transition. -
Tissue specificity
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. -
Sequence similarities
Belongs to the cyclin family. Cyclin E subfamily. -
Post-translational
modificationsPhosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 898 Human
- Entrez Gene: 12447 Mouse
- Entrez Gene: 25729 Rat
- Omim: 123837 Human
- SwissProt: P24864 Human
- SwissProt: Q61457 Mouse
- SwissProt: P39949 Rat
- Unigene: 244723 Human
see all -
Alternative names
- CCNE antibody
- Ccne1 antibody
- CCNE1_HUMAN antibody
see all
Images
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Flow Cytometrical analysis of K562 cells labeling Cyclin E1 with ab211342 at 1/200 (green) compared to a negative control (red).
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All lanes : Anti-Cyclin E1 antibody [5F8C5] (ab211342) at 1/500 dilution
Lane 1 : HeLa cell lysates
Lane 2 : K562 cell lysates
Lane 3 : NIH3T3 cell lysates
Lane 4 : C6 cell lysates
Lane 5 : MCF7 cell lysates
Lane 6 : Jurkat cell lysates
Lane 7 : A431 cell lysates
Predicted band size: 47 kDa -
All lanes : Anti-Cyclin E1 antibody [5F8C5] (ab211342) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Cyclin E1 (AA: 307-410)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 47 kDa -
Anti-Cyclin E1 antibody [5F8C5] (ab211342) at 1/500 dilution + human Cyclin E1 (AA: 307-410) recombinant protein
Predicted band size: 47 kDa
Observed band size: 37.5 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab211342 has been referenced in 1 publication.
- Song Y et al. Thioridazine hydrochloride: an antipsychotic agent that inhibits tumor growth and lung metastasis in triple-negative breast cancer via inducing G0/G1 arrest and apoptosis. Cell Cycle 19:3521-3533 (2020). PubMed: 33315498