Key features and details
- Rabbit polyclonal to Cyclin E1
- Suitable for: Functional Studies, ICC/IF, IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cyclin E1 antibody
See all Cyclin E1 primary antibodies
DescriptionRabbit polyclonal to Cyclin E1
Tested applicationsSuitable for: Functional Studies, ICC/IF, IP, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human Cyclin E1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P24864
- SKBR3 and MAD109 cells. Breast carcinoma or placenta.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
Tissue specificityHighly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Sequence similaritiesBelongs to the cyclin family. Cyclin E subfamily.
modificationsPhosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CCNE antibody
- Ccne1 antibody
- CCNE1_HUMAN antibody
ab74276, at 1/50 dilution, staining Cyclin E in formalin-fixed, paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
ab74276 has not yet been referenced specifically in any publications.