Overview

  • Product name

    Anti-Cyclin E1 antibody [CCNE1/2460]
    See all Cyclin E1 primary antibodies
  • Description

    Mouse monoclonal [CCNE1/2460] to Cyclin E1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, PepArrmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Cyclin E1 aa 10-176.
    Database link: P24864

  • Positive control

    • IHC-P: Human colon carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab238081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at room temperature.

PepArr Use at an assay dependent concentration.

Target

  • Function

    Essential for the control of the cell cycle at the G1/S (start) transition.
  • Tissue specificity

    Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin E subfamily.
  • Post-translational
    modifications

    Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cyclin E1 using ab238081 at 2 µg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cyclin E1 using ab238081 at 2 µg/ml in immunohistochemical analysis.

  • Analysis of Protein Array containing more than 19,000 full-length human proteins using ab238081.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

References

ab238081 has not yet been referenced specifically in any publications.

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