Overview

  • Product name
    Anti-Cyclin E1 antibody [HE12]
    See all Cyclin E1 primary antibodies
  • Description
    Mouse monoclonal [HE12] to Cyclin E1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin E1.

  • Positive control
    • NIH3T3 cells
  • General notes

    This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. The Final dilution sould be determined by the user based upon the staining conditions employed.

Properties

Applications

Our Abpromise guarantee covers the use of ab3927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 47 kDa).

Detects a doublet of 50 kDa and a single band of 42 kDa, see Abreviews.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr or IHC-P.
  • Target

    • Function
      Essential for the control of the cell cycle at the G1/S (start) transition.
    • Tissue specificity
      Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • Sequence similarities
      Belongs to the cyclin family. Cyclin E subfamily.
    • Post-translational
      modifications
      Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCNE antibody
      • Ccne1 antibody
      • CCNE1_HUMAN antibody
      • cyclin E variant ex5del antibody
      • cyclin E variant ex7del antibody
      • Cyclin E1 antibody
      • Cyclin Es antibody
      • Cyclin Et antibody
      • CyclinE antibody
      • G1/S specific cyclin E antibody
      • G1/S-specific cyclin-E1 antibody
      see all

    Images

    • Immunoblot analysis of Cyclin E expression in NIH/3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysed by immunoblotting with ab3927. Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysised by immunoblotting with ab3927.

    References

    This product has been referenced in:
    • He Z  et al. MiR-422a regulates cellular metabolism and malignancy by targeting pyruvate dehydrogenase kinase 2 in gastric cancer. Cell Death Dis 9:505 (2018). Read more (PubMed: 29725130) »
    • Coleman KE  et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017). Read more (PubMed: 28475037) »

    See all 19 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HepG2 liver cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    HepG2 liver cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 22 2015

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Mouse Cell lysate - whole cell (Mouse Pancreatic cancer)
    Specification
    Mouse Pancreatic cancer
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 25 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated
    Sample
    Human Tissue sections (endometiral stromal sarcoma)
    Specification
    endometiral stromal sarcoma
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 30 2013

    Thank you for this additional information. One thing to try is using the 1/200 dilution for 1-2 hours room temperature, to see if you can get the target band without getting all the non-specific ones. Please let me know if this does not improve your re...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab3927 with the order number 946601. To check the status of th...

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    Something does seem odd about the western blot data. By itself, the slightly slower migration of the cyclin E band is not that concerning. But together with the apparent insensitivity to cyclin E levels, it appears the new vial of ab3927 you received m...

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    Thank you for your description of the issues and for including the western blot images. So you are expecting cyclin E to levels to increase due to siEmi1_1?

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLA cells)
    Loading amount
    20 µg
    Specification
    HeLA cells
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 25 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    20 µg
    Specification
    HeLa cells
    Treatment
    2mM double Thymidine block
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 24 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cells)
    Specification
    HeLa cells
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 24 2011

    1-10 of 19 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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