Anti-Cyclin E1 antibody [HE12] (ab3927)
- Datasheet
- References (35)
- Protocols
Overview
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Product name
Anti-Cyclin E1 antibody [HE12]
See all Cyclin E1 primary antibodies -
Description
Mouse monoclonal [HE12] to Cyclin E1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Cyclin E1.
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Positive control
- MCF7 whole cell lysate (ab3871) can be used as a positive control in WB. NIH3T3 cells
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General notes
This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. The Final dilution sould be determined by the user based upon the staining conditions employed.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 99% PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
HE12 -
Myeloma
unknown -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Unmasking reagent
Applications
Our Abpromise guarantee covers the use of ab3927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 47 kDa). Detects a doublet of 50 kDa and a single band of 42 kDa, see Abreviews. |
Target
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Function
Essential for the control of the cell cycle at the G1/S (start) transition. -
Tissue specificity
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. -
Sequence similarities
Belongs to the cyclin family. Cyclin E subfamily. -
Post-translational
modificationsPhosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 898 Human
- Omim: 123837 Human
- SwissProt: P24864 Human
- Unigene: 244723 Human
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Alternative names
- CCNE antibody
- Ccne1 antibody
- CCNE1_HUMAN antibody
see all
Images
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![Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)](https://www.abcam.com/ps/products/3/ab3927/Images/ab3927-319310-anti-cyclin-e1-antibody-he12-western-blot.jpg)
Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)All lanes : Anti-Cyclin E1 antibody [HE12] (ab3927) at 1/500 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CCNE1 (Cyclin E1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 47 kDaLanes 1 - 2: Merged signal (red and green). Green - ab3927 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab3927 was shown to recognize CCNE1 in wild-type HAP1 cells as signal was lost at the expected MW in CCNE1 (Cyclin E1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CCNE1 (Cyclin E1) knockout samples were subjected to SDS-PAGE. Ab3927 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)](https://www.abcam.com/ps/products/3/ab3927/Images/ab3927-8419-anti-cyclin-e1-antibody-he12-western-blot.jpg)
Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)Image kindly provided by Tulsiram Prathapam, as part of an abreview.Immunoblot analysis of Cyclin E expression in NIH/3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysed by immunoblotting with ab3927. Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysised by immunoblotting with ab3927.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Chen Y et al. Lost expression of cell adhesion molecule 1 is associated with bladder cancer progression and recurrence and its overexpression inhibited tumor cell malignant behaviors. Oncol Lett 17:2047-2056 (2019). Read more (PubMed: 30719104) »
- Luo J et al. DMBX1 promotes tumor proliferation and regulates cell cycle progression via repressing OTX2-mediated transcription of p21 in lung adenocarcinoma cell. Cancer Lett 453:45-56 (2019). Read more (PubMed: 30928384) »
