Overview

  • Product name

    Anti-Cyclin E1 antibody [HE12]
    See all Cyclin E1 primary antibodies
  • Description

    Mouse monoclonal [HE12] to Cyclin E1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin E1.

  • Positive control

    • MCF7 whole cell lysate (ab3871) can be used as a positive control in WB. NIH3T3 cells
  • General notes

    This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. The Final dilution sould be determined by the user based upon the staining conditions employed.

Properties

Applications

Our Abpromise guarantee covers the use of ab3927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 47 kDa).

Detects a doublet of 50 kDa and a single band of 42 kDa, see Abreviews.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr or IHC-P.
  • Target

    • Function

      Essential for the control of the cell cycle at the G1/S (start) transition.
    • Tissue specificity

      Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • Sequence similarities

      Belongs to the cyclin family. Cyclin E subfamily.
    • Post-translational
      modifications

      Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • CCNE antibody
      • Ccne1 antibody
      • CCNE1_HUMAN antibody
      • cyclin E variant ex5del antibody
      • cyclin E variant ex7del antibody
      • Cyclin E1 antibody
      • Cyclin Es antibody
      • Cyclin Et antibody
      • CyclinE antibody
      • G1/S specific cyclin E antibody
      • G1/S-specific cyclin-E1 antibody
      see all

    Images

    • All lanes : Anti-Cyclin E1 antibody [HE12] (ab3927) at 1/500 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : CCNE1 (Cyclin E1) knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 47 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab3927 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab3927 was shown to recognize CCNE1 in wild-type HAP1 cells as signal was lost at the expected MW in CCNE1 (Cyclin E1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CCNE1 (Cyclin E1) knockout samples were subjected to SDS-PAGE. Ab3927 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunoblot analysis of Cyclin E expression in NIH/3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysed by immunoblotting with ab3927. Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysised by immunoblotting with ab3927.

    References

    This product has been referenced in:

    • Chen Y  et al. Lost expression of cell adhesion molecule 1 is associated with bladder cancer progression and recurrence and its overexpression inhibited tumor cell malignant behaviors. Oncol Lett 17:2047-2056 (2019). Read more (PubMed: 30719104) »
    • Luo J  et al. DMBX1 promotes tumor proliferation and regulates cell cycle progression via repressing OTX2-mediated transcription of p21 in lung adenocarcinoma cell. Cancer Lett 453:45-56 (2019). Read more (PubMed: 30928384) »
    See all 35 Publications for this product

    Customer reviews and Q&As

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    1-9 of 9 Abreviews

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HepG2 liver cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    HepG2 liver cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Apr 22 2015

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Mouse Cell lysate - whole cell (Mouse Pancreatic cancer)
    Specification
    Mouse Pancreatic cancer
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Feb 25 2014

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated
    Sample
    Human Tissue sections (endometiral stromal sarcoma)
    Specification
    endometiral stromal sarcoma
    Permeabilization
    No
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 30 2013

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLA cells)
    Loading amount
    20 µg
    Specification
    HeLA cells
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Aug 25 2011

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    20 µg
    Specification
    HeLa cells
    Treatment
    2mM double Thymidine block
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Feb 24 2011

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cells)
    Specification
    HeLa cells
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Feb 24 2011

    This product is known to not work in this application or species.
    Application
    Flow Cytometry
    Sample
    Human Cell (Breast cancer (MDA-MB-436))
    Specification
    Breast cancer (MDA-MB-436)
    Preparation
    Cell harvesting/tissue preparation method: Trypsin/EDTA for 5 minutes
    Sample buffer: 1x PBS with 5% FCS
    Fixation
    70% ethanol
    Permeabilization
    No
    Gating Strategy
    live (non apoptotic cells) were selected

    Dr. Penelope Ottewell

    Verified customer

    Submitted Nov 25 2008

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Lymphocytes)
    Loading amount
    1.5e+006 cells
    Specification
    Human Lymphocytes
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Apr 07 2008

    Application
    Western blot
    Sample
    Human Cell lysate - other (human ovarian cancer cell lines)
    Specification
    human ovarian cancer cell lines
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5

    Abcam user community

    Verified customer

    Submitted Jun 22 2006

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