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    cyclin-e1-antibody-he12-ab3927.pdf

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Cell Biology Cell Cycle Cyclins
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Validated using a knockout cell line

Anti-Cyclin E1 antibody [HE12] (ab3927)

  • Datasheet
  • SDS
Reviews (9)Q&A (10)References (49)

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Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)
  • Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)

Key features and details

  • Mouse monoclonal [HE12] to Cyclin E1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

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Protein
Product image
Recombinant Human Cyclin E1 protein (ab119719)
Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Assay
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β-Glucuronidase Activity Assay Kit (Fluorometric) (ab234625)

View more associated products

Overview

  • Product name

    Anti-Cyclin E1 antibody [HE12]
    See all Cyclin E1 primary antibodies
  • Description

    Mouse monoclonal [HE12] to Cyclin E1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin E1.

  • Positive control

    • MCF7 whole cell lysate (ab3871) can be used as a positive control in WB. NIH3T3 cells
  • General notes

    This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. The Final dilution sould be determined by the user based upon the staining conditions employed.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.50
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    HE12
  • Myeloma

    unknown
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cyclin E Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cyclins
    • Cyclin E Family
    • Cancer
    • Cell cycle
    • Cyclins
    • Cyclin E family

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Positive Controls

    • ZR-75-1 nuclear extract lysate (ab14916)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human Cyclin E1 protein (ab119719)
  • Unmasking reagent

    • 10x Citrate Buffer pH 6.0 (ab64214)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB (6)
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.
  • Target

    • Function

      Essential for the control of the cell cycle at the G1/S (start) transition.
    • Tissue specificity

      Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • Sequence similarities

      Belongs to the cyclin family. Cyclin E subfamily.
    • Post-translational
      modifications

      Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P24864 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 898 Human
      • Omim: 123837 Human
      • SwissProt: P24864 Human
      • Unigene: 244723 Human
      • Alternative names

        • CCNE antibody
        • Ccne1 antibody
        • CCNE1_HUMAN antibody
        • cyclin E variant ex5del antibody
        • cyclin E variant ex7del antibody
        • Cyclin E1 antibody
        • Cyclin Es antibody
        • Cyclin Et antibody
        • CyclinE antibody
        • G1/S specific cyclin E antibody
        • G1/S-specific cyclin-E1 antibody
        see all

      Images

      • Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)
        Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)
        All lanes : Anti-Cyclin E1 antibody [HE12] (ab3927) at 1/500 dilution

        Lane 1 : Wild-type HAP1 whole cell lysate
        Lane 2 : CCNE1 (Cyclin E1) knockout HAP1 whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Predicted band size: 47 kDa



        Lanes 1 - 2: Merged signal (red and green). Green - ab3927 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.

        ab3927 was shown to recognize CCNE1 in wild-type HAP1 cells as signal was lost at the expected MW in CCNE1 (Cyclin E1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CCNE1 (Cyclin E1) knockout samples were subjected to SDS-PAGE. Ab3927 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)
        Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)Image kindly provided by Tulsiram Prathapam, as part of an abreview.

        Immunoblot analysis of Cyclin E expression in NIH/3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysed by immunoblotting with ab3927. Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysised by immunoblotting with ab3927.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (49)

      Publishing research using ab3927? Please let us know so that we can cite the reference in this datasheet.

      ab3927 has been referenced in 49 publications.

      • Venugopal N  et al. The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts. BMC Mol Cell Biol 21:25 (2020). PubMed: 32293249
      • Mlyczynska E  et al. Apelin and apelin receptor in human placenta: Expression, signalling pathway and regulation of trophoblast JEG-3 and BeWo cells proliferation and cell cycle. Int J Mol Med 45:691-702 (2020). PubMed: 31922236
      • Huang R  et al. The activation of GPER inhibits cells proliferation, invasion and EMT of triple-negative breast cancer via CD151/miR-199a-3p bio-axis. Am J Transl Res 12:32-44 (2020). PubMed: 32051735
      • Al-Wabli RI  et al. New Isatin-Indole Conjugates: Synthesis, Characterization, and a Plausible Mechanism of Their in vitro Antiproliferative Activity. Drug Des Devel Ther 14:483-495 (2020). PubMed: 32099332
      • Wang Y  et al. The role of low density lipoprotein receptor-related protein 11 as a tumor promoter in cervical cancer. Cancer Manag Res 11:8081-8093 (2019). PubMed: 31507330
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review

      Filter by Application

      Filter by Species

      Filter by Ratings

      1-9 of 9 Abreviews

      Western blot abreview for Anti-Cyclin E1 antibody [HE12]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HepG2 liver cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      50 µg
      Specification
      HepG2 liver cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 22 2015

      Western blot abreview for Anti-Cyclin E1 [HE12] antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      20 µg
      Gel Running Conditions
      Reduced Denaturing (15%)
      Sample
      Mouse Cell lysate - whole cell (Mouse Pancreatic cancer)
      Specification
      Mouse Pancreatic cancer
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 25 2014

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cyclin E antibody [HE12]

      Good
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
      Antigen retrieval step
      Heat mediated
      Sample
      Human Tissue sections (endometiral stromal sarcoma)
      Specification
      endometiral stromal sarcoma
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 30 2013

      Western blot abreview for Anti-Cyclin E antibody [HE12]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLA cells)
      Loading amount
      20 µg
      Specification
      HeLA cells
      Gel Running Conditions
      Reduced Denaturing (10)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 25 2011

      Western blot abreview for Anti-Cyclin E antibody [HE12]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLa cells)
      Loading amount
      20 µg
      Specification
      HeLa cells
      Treatment
      2mM double Thymidine block
      Gel Running Conditions
      Reduced Denaturing (12.5%)
      Blocking step
      Milk as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 24 2011

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin E antibody [HE12]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa cells)
      Specification
      HeLa cells
      Fixative
      Methanol
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 24 2011

      Flow Cytometry abreview for Anti-Cyclin E antibody [HE12]

      Good
      Abreviews
      Abreviews
      Application
      Flow Cytometry
      Sample
      Human Cell (Breast cancer (MDA-MB-436))
      Specification
      Breast cancer (MDA-MB-436)
      Preparation
      Cell harvesting/tissue preparation method: Trypsin/EDTA for 5 minutes
      Sample buffer: 1x PBS with 5% FCS
      Fixation
      70% ethanol
      Permeabilization
      No
      Gating Strategy
      live (non apoptotic cells) were selected
      Read More

      Dr. Penelope Ottewell

      Verified customer

      Submitted Nov 25 2008

      Western blot abreview for Anti-Cyclin E antibody [HE12]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Human Lymphocytes)
      Loading amount
      1.5e+006 cells
      Specification
      Human Lymphocytes
      Gel Running Conditions
      Reduced Denaturing (4-12%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 07 2008

      Western blot abreview for Anti-Cyclin E antibody [HE12]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - other (human ovarian cancer cell lines)
      Specification
      human ovarian cancer cell lines
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 22 2006

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