Recombinant
RabMAb

Recombinant Anti-Cyclin E1 antibody [SP146] (ab135380)

Overview

  • Product name

    Anti-Cyclin E1 antibody [SP146]
    See all Cyclin E1 primary antibodies
  • Description

    Rabbit monoclonal [SP146] to Cyclin E1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cyclin E1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P24864

  • Positive control

    • IHC-P: Human colon, and Human placenta tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab135380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at room temperature.

Target

  • Function

    Essential for the control of the cell cycle at the G1/S (start) transition.
  • Tissue specificity

    Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin E subfamily.
  • Post-translational
    modifications

    Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue sections labeling Cyclin E1 with ab135380 at 1/100 dilution (2.60 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer, pH 6.0. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon tissue labeling Cyclin E1 with ab135380 at a 1/100 dilution.

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 07 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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