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Cell Biology Cell Cycle Cyclins
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Anti-Cyclin E1 (phospho T395) antibody (ab52195)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)

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Western blot - Anti-Cyclin E1 (phospho T395) antibody (ab52195)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 (phospho T395) antibody (ab52195)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 (phospho T395) antibody (ab52195)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 (phospho T395) antibody (ab52195)

Key features and details

  • Rabbit polyclonal to Cyclin E1 (phospho T395)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Cyclin E1 protein (ab119719)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Cyclin E1 (phospho T395) antibody
    See all Cyclin E1 primary antibodies
  • Description

    Rabbit polyclonal to Cyclin E1 (phospho T395)
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of Cyclin E1 only when phosphorylated at threonine 395.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin E1 (phospho T395). Immunogen is in the range of aa 361-410
    Database link: P24864

  • Positive control

    • Human colon carcinoma tissue and extracts from Hela cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cyclin E Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cyclins
    • Cyclin E Family
    • Cancer
    • Cell cycle
    • Cyclins
    • Cyclin E family

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
  • Recombinant Protein

    • Recombinant Human Cyclin E1 protein (ab119719)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Application Abreviews Notes
WB
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/50 - 1/100.
ICC/IF (1)
Use a concentration of 1 µg/ml.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/50 - 1/100.
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Essential for the control of the cell cycle at the G1/S (start) transition.
  • Tissue specificity

    Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin E subfamily.
  • Post-translational
    modifications

    Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P24864 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 898 Human
    • Entrez Gene: 12447 Mouse
    • Omim: 123837 Human
    • SwissProt: P24864 Human
    • SwissProt: Q61457 Mouse
    • Unigene: 244723 Human
    • Unigene: 16110 Mouse
    • Alternative names

      • CCNE antibody
      • Ccne1 antibody
      • CCNE1_HUMAN antibody
      • cyclin E variant ex5del antibody
      • cyclin E variant ex7del antibody
      • Cyclin E1 antibody
      • Cyclin Es antibody
      • Cyclin Et antibody
      • CyclinE antibody
      • G1/S specific cyclin E antibody
      • G1/S-specific cyclin-E1 antibody
      see all

    Images

    • Western blot - Anti-Cyclin E1 (phospho T395) antibody (ab52195)
      Western blot - Anti-Cyclin E1 (phospho T395) antibody (ab52195)
      All lanes : Anti-Cyclin E1 (phospho T395) antibody (ab52195) at 1/500 dilution

      All lanes : HeLa cells treated with Paclitaxel 1uM 60 minutes with phospho peptide

      Predicted band size: 50 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 (phospho T395) antibody (ab52195)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 (phospho T395) antibody (ab52195)
      Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using ab52195 at a 1/50 dilution.

      Left image un-treated.

      Right image treated with phosphopeptide.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 (phospho T395) antibody (ab52195)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 (phospho T395) antibody (ab52195)
      Ab52195 staining human normal placenta. Staining is localized to the nucleus.
      Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 (phospho T395) antibody (ab52195)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 (phospho T395) antibody (ab52195)
      ICC/IF image of ab52195 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52195, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab52195? Please let us know so that we can cite the reference in this datasheet.

    ab52195 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin E1 (phospho T395) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Aug 10 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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