Product nameAnti-Cyclin E1 (phospho T77) antibody [EPR2242(2)]
See all Cyclin E1 primary antibodies
DescriptionRabbit monoclonal [EPR2242(2)] to Cyclin E1 (phospho T77)
Specificityab124696 only detects Cyclin E1 phosphorylated at Threonine 77.
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Cyclin E1 (phospho T77). The exact sequence is proprietary.
- JAR cell lysate, treated with Lambda Phosphatase; Human colon tissue.
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.10 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/250 - 1/500.|
FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
Tissue specificityHighly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Sequence similaritiesBelongs to the cyclin family. Cyclin E subfamily.
modificationsPhosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CCNE antibody
- Ccne1 antibody
- CCNE1_HUMAN antibody
All lanes : Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] (ab124696) at 1/1000 dilution
Lane 1 : JAR cell lysate, untreated
Lane 2 : JAR cell lysate, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 47 kDa
The bands at the bottom of the gel show staining by an anti-Cyclin E1 antibody, demonstrating the presence of the protein in both lanes.
ab124696, at 1/100, staining Cyclin E1 (phospho T77) in paraffin-embedded Human colon tissue by Immunohistochemistry.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124696 has not yet been referenced specifically in any publications.