Recombinant
RabMAb

Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] (ab124696)

Overview

  • Product name
    Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)]
    See all Cyclin E1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2242(2)] to Cyclin E1 (phospho T77)
  • Host species
    Rabbit
  • Specificity
    ab124696 only detects Cyclin E1 phosphorylated at Threonine 77.
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Cyclin E1 (phospho T77). The exact sequence is proprietary.

  • Positive control
    • JAR cell lysate, treated with Lambda Phosphatase; Human colon tissue.
  • General notes

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Essential for the control of the cell cycle at the G1/S (start) transition.
    • Tissue specificity
      Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • Sequence similarities
      Belongs to the cyclin family. Cyclin E subfamily.
    • Post-translational
      modifications
      Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCNE antibody
      • Ccne1 antibody
      • CCNE1_HUMAN antibody
      • cyclin E variant ex5del antibody
      • cyclin E variant ex7del antibody
      • Cyclin E1 antibody
      • Cyclin Es antibody
      • Cyclin Et antibody
      • CyclinE antibody
      • G1/S specific cyclin E antibody
      • G1/S-specific cyclin-E1 antibody
      see all

    Images

    • All lanes : Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] (ab124696) at 1/1000 dilution

      Lane 1 : JAR cell lysate, untreated
      Lane 2 : JAR cell lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 47 kDa



      The bands at the bottom of the gel show staining by an anti-Cyclin E1 antibody, demonstrating the presence of the protein in both lanes.
    • ab124696, at 1/100, staining Cyclin E1 (phospho T77) in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124696 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cell (HeLa)
    Permeabilization
    No
    Specification
    HeLa
    Fixative
    Methanol

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Aug 10 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 06 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up