Anti-Cyclin E2 antibody [E142] (ab32103)
- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-Cyclin E2 antibody [E142]
See all Cyclin E2 primary antibodies -
DescriptionRabbit monoclonal [E142] to Cyclin E2
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Host speciesRabbit
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Specificityab32103 recognises Cyclin E2.
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP -
Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide within Human Cyclin E2 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O96020 -
Positive control
- Human breast carcinoma, HeLa cells lysates, Jurkat cell lysate
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone numberE142
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab32103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| WB | 1/500. Predicted molecular weight: 45 kDa. | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/2000. For unpurified use at 1/100 - 1/250. |
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| Flow Cyt | 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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RelevanceThe human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 9134 Human
- Entrez Gene: 12448 Mouse
- Omim: 603775 Human
- SwissProt: O96020 Human
- SwissProt: Q7TMS8 Mouse
- SwissProt: Q9Z238 Mouse
- Unigene: 567387 Human
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Alternative names
- CCN E2 antibody
- CCNE 2 antibody
- CCNE2 antibody
see all
Images
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![Western blot - Anti-Cyclin E2 antibody [E142] (ab32103)](https://www.abcam.com/ps/products/32/ab32103/Images/Wb cyclin e2.jpg)
Western blot - Anti-Cyclin E2 antibody [E142] (ab32103)Anti-Cyclin E2 antibody [E142] (ab32103) at 1/500 dilution + HeLa cell lysate
Predicted band size: 45 kDa
Observed band size: 50 kDa (why is the actual band size different from the predicted?) -
![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)](https://www.abcam.com/ps/products/32/ab32103/Images/ab32103-261975-ab32103-jurkat-2000.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling Cyclin E2 with purified ab32103 at 1/2000. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)](https://www.abcam.com/ps/products/32/ab32103/Images/IF cyclin e2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)Immunofluorescent analysis of Cyclin E2 expression in HeLa cell culture using 1/100 ab32103. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E2 antibody [E142] (ab32103)](https://www.abcam.com/ps/products/32/ab32103/Images/IHC cyclin e2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E2 antibody [E142] (ab32103)Immunohistochemical analysis of Cyclin E2 expression in paraffin embedded human breast carcinoma using 1/50 ab32103. -
![Flow Cytometry - Anti-Cyclin E2 antibody [E142] (ab32103)](https://www.abcam.com/ps/products/32/ab32103/Images/Cyclin-E2-Primary-antibodies-ab32103-23.jpg)
Flow Cytometry - Anti-Cyclin E2 antibody [E142] (ab32103)Overlay histogram showing HeLa cells stained with ab32103 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32103, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)](https://www.abcam.com/ps/products/32/ab32103/Images/ab32103-300230-ab3210360983.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)This image is courtesy of an abreview by Kirk Mcmanusab32103 staining Cyclin E2 in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. Ab150081 (1/200) was used as the secondary antibody.
References
This product has been referenced in:
- Vannini I et al. Transcribed ultraconserved region 339 promotes carcinogenesis by modulating tumor suppressor microRNAs. Nat Commun 8:1801 (2017). Read more (PubMed: 29180617) »
- Vogel KU et al. The RNA-Binding Proteins Zfp36l1 and Zfp36l2 Enforce the Thymic ß-Selection Checkpoint by Limiting DNA Damage Response Signaling and Cell Cycle Progression. J Immunol 197:2673-85 (2016). Read more (PubMed: 27566829) »
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