Recombinant
RabMAb

Recombinant Anti-Cyclin E2 antibody [EP454Y] (ab40890)

Overview

  • Product name

    Anti-Cyclin E2 antibody [EP454Y]
    See all Cyclin E2 primary antibodies
  • Description

    Rabbit monoclonal [EP454Y] to Cyclin E2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cyclin E2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899). ICC/IF: HeLa cells. IHC-P: Human breast carcinoma. FCt: HeLa cells. IP: Jurkat whole cell lysate.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab40890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20.
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100 - 1/500.

See IHC antigen retrieval protocols.

ICC/IF 1/50 - 1/250.
IP 1/20 - 1/50.

Target

  • Relevance

    The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • CCN E2 antibody
    • CCNE 2 antibody
    • CCNE2 antibody
    • CCNE2 protein antibody
    • CYC E2 antibody
    • CYCE 2 antibody
    • CYCE2 antibody
    • CyclinE2 antibody
    • G1/S specific cyclin E2 antibody
    see all

Images

  • Anti-Cyclin E2 antibody [EP454Y] (ab40890) at 1/1000 dilution (Purified) + Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling Cyclin E2 with purified ab40890 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • ab40890 (purified) at 1/20 dilution (1 µg) immunoprecipitating Cyclin E2 in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 µg
    Lane 2 (+): ab40890 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40890 in Jurkat whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cyclin E2 with purified ab40890 at 1:50 dilution (2.8 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Anti-Cyclin E2 antibody [EP454Y] (ab40890) at 1/2000 dilution (unpurified) + Jurkat cell lysate at 10 µg

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

  • Immunofluorescent staining of staining HeLa cells using ab40890 (unpurified) at 1/100.

  • ab40890 (unpurified) at 1/250 staining human breast carcinoma by immunohistochemistry, paraffin-embedded tissue.

  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cyclin E2 (right) with purified ab40890 at a 1/20 dilution. Cells were fixed with 90% ethanol and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution.

    Left panel - Rabbit monoclonal IgG (ab172730).

References

This product has been referenced in:

  • Tormo E  et al. The miRNA-449 family mediates doxorubicin resistance in triple-negative breast cancer by regulating cell cycle factors. Sci Rep 9:5316 (2019). Read more (PubMed: 30926829) »
  • Ke XX  et al. Knockdown of arsenic resistance protein 2 inhibits human glioblastoma cell proliferation through the MAPK/ERK pathway. Oncol Rep 40:3313-3322 (2018). Read more (PubMed: 30542699) »
See all 15 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 06 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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