Product nameAnti-Cyclin E2 antibody [EP454Y]
See all Cyclin E2 primary antibodies
DescriptionRabbit monoclonal [EP454Y] to Cyclin E2
Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Cyclin E2 aa 100-200. The exact sequence is proprietary.
- WB: Jurkat whole cell lysate (ab7899). ICC/IF: HeLa cells. IHC-P: Human breast carcinoma. Flow Cyt: HeLa cells.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab40890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||1/250 - 1/500.|
|ICC/IF||1/100 - 1/250.|
RelevanceThe human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.
- CCN E2 antibody
- CCNE 2 antibody
- CCNE2 antibody
Anti-Cyclin E2 antibody [EP454Y] (ab40890) at 1/2000 dilution + Jurkat cell lysate at 10 µg
Predicted band size: 50 kDa
Observed band size: 50 kDa
Immunofluorescent staining of staining HeLa cells using ab40890 (1/100).
ab40890 (1/250) staining human breast carcinoma by immunohistochemistry, paraffin-embedded tissue.
Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cyclin E2 (right) with ab40890 at a 1/230 dilution. Cells were fixed with 90% ethanol and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution.
Left panel - Rabbit monoclonal IgG (ab172730).
This product has been referenced in:
- Song W et al. PARP inhibitor increases chemosensitivity by upregulating miR-664b-5p in BRCA1-mutated triple-negative breast cancer. Sci Rep 7:42319 (2017). Read more (PubMed: 28176879) »
- Fischer MM et al. WNT antagonists exhibit unique combinatorial antitumor activity with taxanes by potentiating mitotic cell death. Sci Adv 3:e1700090 (2017). Read more (PubMed: 28691093) »