Recombinant
RabMAb

Recombinant Anti-Cyclin E2 antibody [EP454Y] (HRP) (ab200425)

Overview

  • Product name

    Anti-Cyclin E2 antibody [EP454Y] (HRP)
    See all Cyclin E2 primary antibodies
  • Description

    Rabbit monoclonal [EP454Y] to Cyclin E2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cyclin E2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab200425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • Relevance

    The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • CCN E2 antibody
    • CCNE 2 antibody
    • CCNE2 antibody
    • CCNE2 protein antibody
    • CYC E2 antibody
    • CYCE 2 antibody
    • CYCE2 antibody
    • CyclinE2 antibody
    • G1/S specific cyclin E2 antibody
    see all

Images

  • Anti-Cyclin E2 antibody [EP454Y] (HRP) (ab200425) at 1/5000 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab200425 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab200425 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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