The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/50 - 1/100. Predicted molecular weight: 34 kDa.
May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation.
High levels in skeletal muscle, ovary, kidney and colon.
Belongs to the cyclin family. Cyclin G subfamily.
Very low levels in normal cells during G1 phase, which increase as cells enter the S phase and stay high throughout the S and G2/M phases. In breast cancer cells consistent high levels are found throughout the cell cycle.
Flow Cytometry - Anti-Cyclin G antibody - C-terminal (ab170389)
Flow cytometric analysis of HeLa cells labeling Cyclin G with ab170389 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC conjugated Goat anti-rabbit secondary antibodies were used for the analysis.
Western blot - Anti-Cyclin G antibody - C-terminal (ab170389)
Anti-Cyclin G antibody - C-terminal (ab170389) at 1/50 dilution + Mouse kidney tissue lysates at 35 µg