Key features and details
- Rabbit polyclonal to Cyclin T1
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cyclin T1 antibody
See all Cyclin T1 primary antibodies
DescriptionRabbit polyclonal to Cyclin T1
Tested applicationsSuitable for: IHC-P, IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa and 293T whole cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 81 kDa.|
FunctionRegulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the cyclin family. Cyclin C subfamily.
- Information by UniProt
- CCN T1 antibody
- CCNT antibody
- CCNT 1 antibody
All lanes : Anti-Cyclin T1 antibody (ab176702) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Predicted band size: 81 kDa
Exposure time: 3 minutes
Detection of Cyclin T1 by Western Blot of Immunprecipitate.
Lane 1: ab176702 at 0.04µg/ml labeling Cyclin T1 in HeLa whole cell lysate immunoprecipitated using ab176702 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 3 seconds.
ab176702 has been referenced in 1 publication.
- Mück F et al. DDX6 transfers P-TEFb kinase to the AF4/AF4N (AFF1) super elongation complex. Am J Blood Res 6:28-45 (2016). PubMed: 27679741