Overview

  • Product name

  • Description

    Rabbit polyclonal to Cyclin T1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment within Human Cyclin T1 (internal sequence). The exact sequence is proprietary.
    Database link: O60563

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab226851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 81 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin C subfamily.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCN T1 antibody
    • CCNT antibody
    • CCNT 1 antibody
    • Ccnt1 antibody
    • CCNT1_HUMAN antibody
    • CDK9 associated C type protein antibody
    • Cyc T1 antibody
    • Cyclin C related protein antibody
    • Cyclin T antibody
    • cyclin T1 antibody
    • Cyclin T1b antibody
    • Cyclin-T antibody
    • Cyclin-T1 antibody
    • CYCT 1 antibody
    • CycT1 antibody
    • HIVE1 antibody
    • Human immunodeficiency virus 1 expression antibody
    • Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1 antibody
    • pTEFb subunit antibody
    • Subunit of positive elongation transcription factor b antibody
    see all

Images

  • All lanes : Anti-Cyclin T1 antibody (ab226851) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 81 kDa



    7.5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cyclin T1 (green) using ab226851 at 1/500 dilution in ICC/IF. Red: phalloidin, a cytoskeleton marker at 1/100 dilution.

References

ab226851 has not yet been referenced specifically in any publications.

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