Product nameAnti-Cyclin T1 antibody
See all Cyclin T1 primary antibodies
DescriptionRabbit polyclonal to Cyclin T1
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide within Human Cyclin T1 aa 325-375. The exact sequence is proprietary. NP_001231.2
Database link: O60563
- IHC-P: Human breast carcinoma tissue. WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/100 - 1/500.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 81 kDa.|
FunctionRegulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the cyclin family. Cyclin C subfamily.
- Information by UniProt
- CCN T1 antibody
- CCNT antibody
- CCNT 1 antibody
All lanes : Anti-Cyclin T1 antibody (ab264325) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T whole cell lysate at 50 µg
Predicted band size: 81 kDa
Exposure time: 30 seconds
Cyclin T1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264325 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264325 at 1 µg/ml.
Lane 1: ab264325 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling Cyclin T1 with ab264325 at 1/200 dilution. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264325 has not yet been referenced specifically in any publications.