Anti-Cyclin T1 antibody (ab264325)
Key features and details
- Rabbit polyclonal to Cyclin T1
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Cyclin T1 antibody
See all Cyclin T1 primary antibodies -
Description
Rabbit polyclonal to Cyclin T1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Synthetic peptide within Human Cyclin T1 aa 325-375. The exact sequence is proprietary. NP_001231.2
Database link: O60563 -
Positive control
- IHC-P: Human breast carcinoma tissue. WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at 2-10 µg/mg of lysate.
|
|
IHC-P |
1/100 - 1/500.
|
|
WB |
1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
|
Notes |
---|
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/100 - 1/500. |
WB
1/2000 - 1/10000. Predicted molecular weight: 81 kDa. |
Target
-
Function
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the cyclin family. Cyclin C subfamily. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 450146 Chimpanzee
- Entrez Gene: 904 Human
- Omim: 143055 Human
- SwissProt: Q8HXN7 Chimpanzee
- SwissProt: O60563 Human
- Unigene: 279906 Human
-
Alternative names
- CCN T1 antibody
- CCNT antibody
- CCNT 1 antibody
see all
Images
-
All lanes : Anti-Cyclin T1 antibody (ab264325) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T whole cell lysate at 50 µg
Predicted band size: 81 kDa
Exposure time: 30 seconds4-8% SDS-PAGE.
-
Cyclin T1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264325 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264325 at 1 µg/ml.
Lane 1: ab264325 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 secs.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling Cyclin T1 with ab264325 at 1/200 dilution. Detection: DAB.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab264325 has not yet been referenced specifically in any publications.