Product nameAnti-Cyclin T1 antibody
See all Cyclin T1 primary antibodies
DescriptionSheep polyclonal to Cyclin T1
Tested applicationsSuitable for: WB, IP, EMSAmore details
Species reactivityReacts with: Mouse, Horse, Human, Monkey
Does not react with: Drosophila melanogaster
Recombinant fragment, corresponding to C terminal amino acids 291-726 of Human Cyclin T1
ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Primary antibody notesab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.
Our Abpromise guarantee covers the use of ab27963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 81 kDa).|
|IP||Use at an assay dependent concentration.|
|EMSA||Use at an assay dependent concentration. PubMed: 17576689|
FunctionRegulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the cyclin family. Cyclin C subfamily.
- Information by UniProt
- CCN T1 antibody
- CCNT antibody
- CCNT 1 antibody
All lanes : Anti-Cyclin T1 antibody (ab27963) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa cell nuclear lysate
Lane 3 : monkey Vero cell lysate
Lane 4 : mouse kidney cell lysate
Lane 5 : Drosophila S2 cell lysate
Lane 6 : horse dermal cell lysate
All lanes : donkey anti-sheep peroxidase conjugate at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Brogie JE & Price DH Reconstitution of a functional 7SK snRNP. Nucleic Acids Res 45:6864-6880 (2017). Read more (PubMed: 28431135) »
- Kuzmina A et al. Super elongation complex promotes early HIV transcription and its function is modulated by P-TEFb. Transcription 8:133-149 (2017). Read more (PubMed: 28340332) »