Product nameAnti-Cyclin T2 antibody [2128C1a]
See all Cyclin T2 primary antibodies
DescriptionMouse monoclonal [2128C1a] to Cyclin T2
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment designed around the C-terminal end of human Cyclin T2.
Epitopeab50979 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
General notesThis antibody recognizes Cyclin T2a and T2b since it is made by immunogen-peptide that is common domain from both protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab50979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 81 kDa.|
|Dot blot||Use at an assay dependent dilution.|
FunctionRegulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II).
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the cyclin family. Cyclin C subfamily.
- Information by UniProt
- CCNT2 antibody
- CCNT2_HUMAN antibody
- Cyclin T2a antibody
Anti-Cyclin T2 antibody [2128C1a] (ab50979) + recombinant protein fragment used as immunogen.
Diagram underneath blot depicts region of Cyclin T2 protein that was employed as immunogen (arrow).
Predicted band size: 81 kDa
All lanes : Anti-Cyclin T2 antibody [2128C1a] (ab50979)
Lane 1 : Molecular Weight Marker
Lane 2 : 50µg Hela Whole cell lysate
Lane 3 : 50µg HT-1080 Whole cell lysate
Lane 4 : 50 µg HEK293 Whole cell lysate
Lane 5 : 50µg A2058 Whole cell lysate
Lane 6 : 50µg HIH3T3 Whole cell Lysate
Lanes 2-6 : Mouse IgG Antibody 1/2500
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Exposure time: 1 minute
8% SDS-PAGE gel;
a- Isoform a = 74kDa (663 a.a.);
b - Isoform b = 81kDa (730 a.a.)
This product has been referenced in:
- Zhang H et al. SIRT2 directs the replication stress response through CDK9 deacetylation. Proc Natl Acad Sci U S A 110:13546-51 (2013). Read more (PubMed: 23898190) »
- Wang XS et al. MicroRNA-29a and microRNA-142-3p are regulators of myeloid differentiation and acute myeloid leukemia. Blood 119:4992-5004 (2012). WB ; Human . Read more (PubMed: 22493297) »