Overview

  • Product name

    Anti-Cyclin T2/CCNT2 antibody
    See all Cyclin T2/CCNT2 primary antibodies
  • Description

    Rabbit polyclonal to Cyclin T2/CCNT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Cyclin T2/CCNT2 aa 497-707.

  • Positive control

    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: Hela cell IHC-P: H1299 Xenograft; mouse brain tissue.
  • General notes

     This product was previously labelled as Cyclin T2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab96133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 81 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II).
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the cyclin family. Cyclin C subfamily.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCNT2 antibody
    • CCNT2_HUMAN antibody
    • Cyclin T2a antibody
    • Cyclin T2b antibody
    • Cyclin-T2 antibody
    • CycT2 antibody
    • FLJ90560 antibody
    • MGC134840 antibody
    • SDS stable vimentin bound DNA fragment HEF42VIM22 antibody
    • Subunit of positive elongation transcription factor b antibody
    see all

Images

  • ab96133, at a 1/100 dilution, staining Cyclin T2/CCNT2 in paraffin embedded mouse brain tissue by Immunohistochemistry.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Anti-Cyclin T2/CCNT2 antibody (ab96133) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 81 kDa



    7.5% SDS PAGE
  • ab96133, at a 1/200 dilution, staining Cyclin T2/CCNT2 in paraformaldehyde fixed A431 cells by Immunofluorescence.
    Lower image: merged with DNA probe.

  • ab96133, at a 1/100 dilution, staining Cyclin T2/CCNT2 in paraffin embedded H1299 xenograft by Immunohistochemistry.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

References

This product has been referenced in:

  • Oqani RK  et al. P-TEFb Kinase Activity Is Essential for Global Transcription, Resumption of Meiosis and Embryonic Genome Activation in Pig. PLoS One 11:e0152254 (2016). Read more (PubMed: 27011207) »
See 1 Publication for this product

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