Key features and details
- Rabbit polyclonal to Cyclin Y
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cyclin Y antibody
See all Cyclin Y primary antibodies
DescriptionRabbit polyclonal to Cyclin Y
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Cyclin Y aa 2-170.
Database link: Q8ND76
- WB: K562 whole cell lysate. IHC-P: Human liver tissue. ICC/IF: HepG2 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/5000. Predicted molecular weight: 39 kDa.|
|ICC/IF||1/200 - 1/500.|
RelevanceCyclin Y is a cyclin which plays a role in the control of cell division cycles and regulation of cyclin dependent kinases (e.g., CDC2; MIM 116940). Single nucleotide polymorphism in Cyclin Y gene is associated with Crohn's disease and ulcerative colitis.
Cellular localizationCell membrane; Lipid-anchor; Cytoplasmic side. Isoform 3: Nucleus
- C10orf9 antibody
- CBCP1 antibody
- CCNX antibody
Anti-Cyclin Y antibody (ab237677) at 1/500 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Paraffin-embedded human liver tissue stained for Cyclin Y using ab237677 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Cyclin Y (green) using ab237677 at 1/200 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237677 has not yet been referenced specifically in any publications.