Overview

  • Product name

  • Description

    Rabbit polyclonal to Cyclin Y
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Cyclin Y aa 2-170.
    Database link: Q8ND76

  • Positive control

    • WB: K562 whole cell lysate. IHC-P: Human liver tissue. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab237677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/5000. Predicted molecular weight: 39 kDa.
ICC/IF 1/200 - 1/500.

Target

  • Relevance

    Cyclin Y is a cyclin which plays a role in the control of cell division cycles and regulation of cyclin dependent kinases (e.g., CDC2; MIM 116940). Single nucleotide polymorphism in Cyclin Y gene is associated with Crohn's disease and ulcerative colitis.
  • Cellular localization

    Cell membrane; Lipid-anchor; Cytoplasmic side. Isoform 3: Nucleus
  • Database links

  • Alternative names

    • C10orf9 antibody
    • CBCP1 antibody
    • CCNX antibody
    • CCNY antibody
    • CFP1 antibody
    • Chromosome 10 open reading frame 9 antibody
    • Cyclin box carrying protein 1 antibody
    • Cyclin box protein 1 antibody
    • Cyclin fold protein 1 antibody
    • Cyclin X antibody
    • Cyclin Y antibody
    see all

Images

  • Anti-Cyclin Y antibody (ab237677) at 1/500 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 39 kDa

  • Paraffin-embedded human liver tissue stained for Cyclin Y using ab237677 at 1/600 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Cyclin Y (green) using ab237677 at 1/200 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. 

References

ab237677 has not yet been referenced specifically in any publications.

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