Cycloheximide, Protein synthesis inhibitor (ab120093)
Key features and details
- Protein synthesis inhibitor
- CAS Number: 66-81-9
- Soluble in water to 25 mM
- Form / State: Solid
- Source: Synthetic
Overview
-
Product name
Cycloheximide, Protein synthesis inhibitor -
Description
Protein synthesis inhibitor -
Biological description
Protein synthesis inhibitor. Antifungal antibiotic.
-
CAS Number
66-81-9 -
Chemical structure
Properties
-
Chemical name
3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide -
Molecular weight
281.35 -
Molecular formula
C15H23NO4 -
PubChem identifier
6197 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 25 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES
O=C2CC(C[C@@H](O)[C@@H]1C[C@@H](C)C[C@H](C)C1=O)CC(=O)N2 -
Source
Synthetic
-
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
Images
-
Chemical Structure - Cycloheximide, Protein synthesis inhibitor (ab120093)2D chemical structure image of ab120093, Cycloheximide, Protein synthesis inhibitor
-
Functional Studies - Cycloheximide, Protein synthesis inhibitor (ab120093)Functional assays: Caspase 8 (active) FITC Staining Kit (ab65614)
Active caspase 8 in control Jurkat cells (10e6/mL) or cells treated for five hours with 10 ug/mL Cyclohexamide (CHX) (ab120093) or four hours with 25 ug/mL Mitomycin C (MitoC) (ab120797). Background signal subtracted, duplicates; +/- SD.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (17)
ab120093 has been referenced in 17 publications.
- Mancini A et al. LGR4 deficiency results in delayed puberty through impaired Wnt/ß-catenin signaling. JCI Insight 5:N/A (2020). PubMed: 32493844
- Nilchian A et al. CXADR-Mediated Formation of an AKT Inhibitory Signalosome at Tight Junctions Controls Epithelial-Mesenchymal Plasticity in Breast Cancer. Cancer Res 79:47-60 (2019). PubMed: 30385615
- Chen J et al. Heat shock protein 47 (HSP47) binds to discoidin domain-containing receptor 2 (DDR2) and regulates its protein stability. J Biol Chem 294:16846-16854 (2019). PubMed: 31570520
- Zhao S et al. BAG3 promotes autophagy and glutaminolysis via stabilizing glutaminase. Cell Death Dis 10:284 (2019). PubMed: 30910998
- Zhou G et al. Tyrosine nitrations impaired intracellular trafficking of FSHR to the cell surface and FSH-induced Akt-FoxO3a signaling in human granulosa cells. Aging (Albany NY) 11:3094-3116 (2019). PubMed: 31097679