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    cycloheximide-protein-synthesis-inhibitor-ab120093.pdf

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Biochemicals Product Range Just Add Water
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Cycloheximide, Protein synthesis inhibitor (ab120093)

  • Datasheet
  • SDS
  • COA
Submit a review Q&A (1)References (22)

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Chemical Structure - Cycloheximide, Protein synthesis inhibitor (ab120093)
  • Functional Studies - Cycloheximide, Protein synthesis inhibitor (ab120093)

Key features and details

  • Protein synthesis inhibitor
  • CAS Number: 66-81-9
  • Soluble in water to 25 mM
  • Form / State: Solid
  • Source: Synthetic

Overview

  • Product name

    Cycloheximide, Protein synthesis inhibitor
  • Description

    Protein synthesis inhibitor
  • Biological description

    Protein synthesis inhibitor. Antifungal antibiotic.

  • CAS Number

    66-81-9
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide
  • Molecular weight

    281.35
  • Molecular formula

    C15H23NO4
  • PubChem identifier

    6197
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water to 25 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

     Toxic, refer to SDS for further information.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    O=C2CC(C[C@@H](O)[C@@H]1C[C@@H](C)C[C@H](C)C1=O)CC(=O)N2
  • Source

    Synthetic

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Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab120093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Notes
Functional Studies
Use at an assay dependent concentration.

Images

  • Chemical Structure - Cycloheximide, Protein synthesis inhibitor (ab120093)
    Chemical Structure - Cycloheximide, Protein synthesis inhibitor (ab120093)
    2D chemical structure image of ab120093, Cycloheximide, Protein synthesis inhibitor
  • Functional Studies - Cycloheximide, Protein synthesis inhibitor (ab120093)
    Functional Studies - Cycloheximide, Protein synthesis inhibitor (ab120093)

    Functional assays: Caspase 8 (active) FITC Staining Kit (ab65614)

    Active caspase 8 in control Jurkat cells (10e6/mL) or cells treated for five hours with 10 ug/mL Cyclohexamide (CHX) (ab120093) or four hours with 25 ug/mL Mitomycin C (MitoC) (ab120797). Background signal subtracted, duplicates; +/- SD.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (22)

Publishing research using ab120093? Please let us know so that we can cite the reference in this datasheet.

ab120093 has been referenced in 22 publications.

  • Xu S  et al. MicroRNA-200c-targeted contactin 1 facilitates the replication of influenza A virus by accelerating the degradation of MAVS. PLoS Pathog 18:e1010299 (2022). PubMed: 35171955
  • Chu Y  et al. LUBAC and OTULIN regulate autophagy initiation and maturation by mediating the linear ubiquitination and the stabilization of ATG13. Autophagy 17:1684-1699 (2021). PubMed: 32543267
  • Xu C  et al. ATE1 Inhibits Liver Cancer Progression through RGS5-Mediated Suppression of Wnt/ß-Catenin Signaling. Mol Cancer Res 19:1441-1453 (2021). PubMed: 34158395
  • Gao G  et al. TGF-ß1 Facilitates TAp63a Protein Lysosomal Degradation to Promote Pancreatic Cancer Cell Migration. Biology (Basel) 10:N/A (2021). PubMed: 34203341
  • Li W  et al. SARS-CoV-2 Nsp5 Activates NF-?B Pathway by Upregulating SUMOylation of MAVS. Front Immunol 12:750969 (2021). PubMed: 34858407
View all Publications for this product

Customer reviews and Q&As

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Question

Phone call requesting information on how to open and how to handle Cycloheximide (ab120093).

Read More

Abcam community

Verified customer

Asked on Apr 02 2012

Answer

Thank you for contacting us on Friday.

I have been over to have a look at the pot of Cycloheximide (ab120093) which you would have received. The compound comes in a tamper safe vial, which should have come with a light blue "key" to open the pot. If you no longer have this key, a strong piece of plastic or metal of about a centimeter and a half should work. Something like a 2 pence coin should work. Simply place on the top of the vial, between the two notches and twist the lid. If you have any problems please do let us know and I'll try to explain it better.

As for handling the compound, the exact precautions that need to be taken to handle it safely can be seen in detail in the MSDS (attached to this email). Oncethe vial has been opened itshould be stored at4 degreesunder desiccating conditions.Itshould notbefrozen.If you do not feel comfortable handling this sort of compound I would suggest asking one of your colleagues in your department (such as the safely officer) for advice.

I hope this information has been of help.If you require any further information please do not hesitate tocontact us again.

Read More

Abcam Scientific Support

Answered on Apr 02 2012

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