Key features and details
- Assay type: Enzyme activity
- Detection method: Luminescent
- Platform: Microplate reader
- Sensitivity: 0.147 U/ml
Product nameCyclooxygenase (COX) Activity Assay Kit (Luminometric)
See all Cyclooxygenase kits
Intra-assay Sample n Mean SD CV% COX 16 30U/ml 7% COX 16 7.5U/ml 3.1%
Assay typeEnzyme activity
Range7.5 U/ml - 60 U/ml
The Cyclooxygenase (COX) Activity Assay Kit (Luminometric) (ab139432) is a non-radioactive COX activity assay. This kit allows user to easily evaluate COX I and COX II inhibitors in a convenient 96-well plate based assay with < 7% intra-assay variation.
COX Activity Assay Kit (Luminometric) uses a specific chemiluminescent substrate to detect the peroxidative activity of COX enzymes. After inhibition by NSAIDs, the direct residual activity of COX is measured by addition of a proprietary luminescent substrate and arachidonic acid. Light emission starts immediately and is directly proportional to the COX activity in the sample. The chemiluminescent signal is measured over 5 seconds.
COX (cyclooxygenase, also known as Prostaglandin G/H synthase) is a membrane-bound enzyme responsible for the oxidation of arachidonic acid to Prostaglandin G2 (PGG2) and the subsequent reduction of PGG2 to PGH2. These reactions are the first steps in the formation of a variety of prostanoids.
COX has been shown to be expressed in at least two different isoforms: a constitutively expressed form, COX1, and an inducible form, COX2. COX1 is thought to regulate a number of "housekeeping" functions such as vascular hemostasis and renal blood flow. Inflammation mediators such as growth factors, cytokines and endotoxin induce COX2 expression in a number of cellular systems.
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests COX Substrate 1 x 10ml Ibuprofen 1 x 2ml Meloxicam, NSAID 1 x 2ml Plate Sealer 2 units White 96 well microtiter plate 1 unit
ab139432 has been referenced in 1 publication.
- Estrada-Figueroa LA et al. Leishmania mexicana gp63 is the enzyme responsible for cyclooxygenase (COX) activity in this parasitic protozoa. Biochimie 151:73-84 (2018). PubMed: 29864507