Overview

  • Product name

    Anti-Cyclophilin 40 antibody
    See all Cyclophilin 40 primary antibodies
  • Description

    Rabbit polyclonal to Cyclophilin 40
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide within Human Cyclophilin 40 aa 175-225. The exact sequence is proprietary.
    Database link: Q08752

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226415 was affinity purified using an epitope specific to Cyclophilin 40 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.
    Contains 1 PPIase cyclophilin-type domain.
    Contains 3 TPR repeats.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 40 kDa peptidyl prolyl cis trans isomerase antibody
    • 40 kDa peptidyl prolyl cis trans isomerase D antibody
    • 40 kDa peptidyl-prolyl cis-trans isomerase antibody
    • Cyclophilin D antibody
    • Cyclophilin related protein antibody
    • Cyclophilin-40 antibody
    • Cyclophilin-related protein antibody
    • Cyclophilin40 antibody
    • CyclophilinD antibody
    • CYP 40 antibody
    • CYP-40 antibody
    • CYP40 antibody
    • CYPD antibody
    • MGC33096 antibody
    • Peptidyl Prolyl Isomerase D antibody
    • Peptidyl-prolyl cis-trans isomerase D antibody
    • Peptidylprolyl isomerase D antibody
    • PPIase antibody
    • PPIase D antibody
    • Ppid antibody
    • PPID_HUMAN antibody
    • Rotamase antibody
    • Rotamase D antibody
    see all

Images

  • All lanes : Anti-Cyclophilin 40 antibody (ab226415) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 41 kDa


    Exposure time: 3 seconds


    Lysates prepared using NETN lysis buffer.

  • Cyclophilin 40 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226415 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226415 at 0.4 µg/ml.

    Lane 1: ab226415 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab226415 has not yet been referenced specifically in any publications.

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