Product nameAnti-Cyclophilin 40 antibody
See all Cyclophilin 40 primary antibodies
DescriptionRabbit polyclonal to Cyclophilin 40
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide within Human Cyclophilin 40 aa 175-225. The exact sequence is proprietary.
Database link: Q08752
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226415 was affinity purified using an epitope specific to Cyclophilin 40 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 41 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the cyclophilin-type PPIase family. PPIase D subfamily.
Contains 1 PPIase cyclophilin-type domain.
Contains 3 TPR repeats.
- Information by UniProt
- 40 kDa peptidyl prolyl cis trans isomerase antibody
- 40 kDa peptidyl prolyl cis trans isomerase D antibody
- 40 kDa peptidyl-prolyl cis-trans isomerase antibody
All lanes : Anti-Cyclophilin 40 antibody (ab226415) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 3 seconds
Lysates prepared using NETN lysis buffer.
Cyclophilin 40 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226415 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226415 at 0.4 µg/ml.
Lane 1: ab226415 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab226415 has not yet been referenced specifically in any publications.