Anti-Cyclophilin 40 antibody (ab3562)
Key features and details
- Rabbit polyclonal to Cyclophilin 40
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
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- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cyclophilin 40 antibody
See all Cyclophilin 40 primary antibodies -
Description
Rabbit polyclonal to Cyclophilin 40 -
Host species
Rabbit -
Specificity
Detects cyclophilin 40 (CyP 40) from Human and Rat tissues and cells. This antibody does not cross-react with CyPA. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Cyclophilin 40 aa 356-370.
Sequence:AQKDKEKAVYAKMFA
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Proprietary Purification -
Primary antibody notes
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP 40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP 18) and FKBP59. CyP 40 exhibits the characteristic CsA binding and isomerase activity of CyP 18, though these activities appear to be less with CyP 40 than with Cyp 18. Like FKBP59, CyP 40 has been found in progesterone receptor complexes. CyP 40 is expressed at similar levels in many tissues. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
1/200.
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Notes |
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WB
1/1000. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/200. |
Target
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Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.
Contains 1 PPIase cyclophilin-type domain.
Contains 3 TPR repeats. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5481 Human
- Entrez Gene: 361967 Rat
- Omim: 601753 Human
- SwissProt: Q08752 Human
- SwissProt: Q6DGG0 Rat
- Unigene: 183958 Human
- Unigene: 136915 Rat
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Alternative names
- 40 kDa peptidyl prolyl cis trans isomerase antibody
- 40 kDa peptidyl prolyl cis trans isomerase D antibody
- 40 kDa peptidyl-prolyl cis-trans isomerase antibody
see all
Images
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ab3562 at a dilution of 1/1000 staining Cyp 40 in Rat spleen lysate by Western blot.
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Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling Cyclophilin 40 (green) with ab3562 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/Immunofluorescence analysis of A431 cells labeling Cyclophilin 40 (green) with ab3562 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human hepatocarcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at aab3562) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/100 with a rabbit polyclonal antibody recognizing Cyclophilin D (ab3562) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab3562 has been referenced in 7 publications.
- Ren Z et al. Knockdown of lncRNA JPX suppresses IL-1β-stimulated injury in chondrocytes through modulating an miR-25-3p/PPID axis. Oncol Lett 24:388 (2022). PubMed: 36276499
- Yim KH et al. Gambogic acid identifies an isoform-specific druggable pocket in the middle domain of Hsp90ß. Proc Natl Acad Sci U S A 113:E4801-9 (2016). PubMed: 27466407
- Zhu G et al. Acute effect of lactic acid on tumor-endothelial cell metabolic coupling in the tumor microenvironment. Oncol Lett 12:3478-3484 (2016). WB ; Human . PubMed: 27900024
- Xu XD et al. The study of energy metabolism in bladder cancer cells in co-culture conditions using a microfluidic chip. Int J Clin Exp Med 8:12327-36 (2015). PubMed: 26550142
- Allan AM et al. Prenatal alcohol exposure modifies glucocorticoid receptor subcellular distribution in the medial prefrontal cortex and impairs frontal cortex-dependent learning. PLoS One 9:e96200 (2014). WB ; Mouse . PubMed: 24755652
- Han J et al. Deregulation of mitochondrial membrane potential by mitochondrial insertion of granzyme B and direct hax-1 cleavage. J Biol Chem 285:22461-72 (2010). WB ; Human . PubMed: 20388708
- Trauernicht AM et al. Modulation of estrogen receptor alpha protein level and survival function by DBC-1. Mol Endocrinol 21:1526-36 (2007). PubMed: 17473282