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Immunology Immune Interventions Transplantation
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Anti-Cyclophilin 40 antibody (ab3562)

  • Datasheet
  • SDS
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Western blot - Anti-Cyclophilin 40 antibody (ab3562)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody (ab3562)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody (ab3562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody (ab3562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody (ab3562)

Key features and details

  • Rabbit polyclonal to Cyclophilin 40
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Recombinant mouse Cyclophilin 40 protein (Active) (ab229174)

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Overview

  • Product name

    Anti-Cyclophilin 40 antibody
    See all Cyclophilin 40 primary antibodies
  • Description

    Rabbit polyclonal to Cyclophilin 40
  • Host species

    Rabbit
  • Specificity

    Detects cyclophilin 40 (CyP 40) from Human and Rat tissues and cells. This antibody does not cross-react with CyPA.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Cyclophilin 40 aa 356-370.
    Sequence:

    AQKDKEKAVYAKMFA

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Primary antibody notes

    Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP 40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP 18) and FKBP59. CyP 40 exhibits the characteristic CsA binding and isomerase activity of CyP 18, though these activities appear to be less with CyP 40 than with Cyp 18. Like FKBP59, CyP 40 has been found in progesterone receptor complexes. CyP 40 is expressed at similar levels in many tissues.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immune Interventions
    • Transplantation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant human Cyclophilin 40 protein (ab78815)
  • Recombinant Protein

    • Recombinant mouse Cyclophilin 40 protein (Active) (ab229174)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Rat
Application Abreviews Notes
WB
1/1000.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/200.
Notes
WB
1/1000.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/200.

Target

  • Function

    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.
    Contains 1 PPIase cyclophilin-type domain.
    Contains 3 TPR repeats.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q08752 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5481 Human
    • Entrez Gene: 361967 Rat
    • Omim: 601753 Human
    • SwissProt: Q08752 Human
    • SwissProt: Q6DGG0 Rat
    • Unigene: 183958 Human
    • Unigene: 136915 Rat
    • Alternative names

      • 40 kDa peptidyl prolyl cis trans isomerase antibody
      • 40 kDa peptidyl prolyl cis trans isomerase D antibody
      • 40 kDa peptidyl-prolyl cis-trans isomerase antibody
      • Cyclophilin D antibody
      • Cyclophilin related protein antibody
      • Cyclophilin-40 antibody
      • Cyclophilin-related protein antibody
      • Cyclophilin40 antibody
      • CyclophilinD antibody
      • CYP 40 antibody
      • CYP-40 antibody
      • CYP40 antibody
      • CYPD antibody
      • MGC33096 antibody
      • Peptidyl Prolyl Isomerase D antibody
      • Peptidyl-prolyl cis-trans isomerase D antibody
      • Peptidylprolyl isomerase D antibody
      • PPIase antibody
      • PPIase D antibody
      • Ppid antibody
      • PPID_HUMAN antibody
      • Rotamase antibody
      • Rotamase D antibody
      see all

    Images

    • Western blot - Anti-Cyclophilin 40 antibody (ab3562)
      Western blot - Anti-Cyclophilin 40 antibody (ab3562)

      ab3562 at a dilution of 1/1000 staining Cyp 40 in Rat spleen lysate by Western blot.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody (ab3562)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody (ab3562)

      Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling Cyclophilin 40 (green) with ab3562 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody (ab3562)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody (ab3562)

      Immunocytochemistry/Immunofluorescence analysis of A431 cells labeling Cyclophilin 40 (green) with ab3562 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody (ab3562)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody (ab3562)

      Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human hepatocarcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at aab3562) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody (ab3562)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody (ab3562)

      Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/100 with a rabbit polyclonal antibody recognizing Cyclophilin D (ab3562) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab3562? Please let us know so that we can cite the reference in this datasheet.

    ab3562 has been referenced in 6 publications.

    • Yim KH  et al. Gambogic acid identifies an isoform-specific druggable pocket in the middle domain of Hsp90ß. Proc Natl Acad Sci U S A 113:E4801-9 (2016). PubMed: 27466407
    • Zhu G  et al. Acute effect of lactic acid on tumor-endothelial cell metabolic coupling in the tumor microenvironment. Oncol Lett 12:3478-3484 (2016). WB ; Human . PubMed: 27900024
    • Xu XD  et al. The study of energy metabolism in bladder cancer cells in co-culture conditions using a microfluidic chip. Int J Clin Exp Med 8:12327-36 (2015). PubMed: 26550142
    • Allan AM  et al. Prenatal alcohol exposure modifies glucocorticoid receptor subcellular distribution in the medial prefrontal cortex and impairs frontal cortex-dependent learning. PLoS One 9:e96200 (2014). WB ; Mouse . PubMed: 24755652
    • Han J  et al. Deregulation of mitochondrial membrane potential by mitochondrial insertion of granzyme B and direct hax-1 cleavage. J Biol Chem 285:22461-72 (2010). WB ; Human . PubMed: 20388708
    • Trauernicht AM  et al. Modulation of estrogen receptor alpha protein level and survival function by DBC-1. Mol Endocrinol 21:1526-36 (2007). PubMed: 17473282

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