Key features and details
- Rabbit polyclonal to Cyclophilin A
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cyclophilin A antibody
See all Cyclophilin A primary antibodies
DescriptionRabbit polyclonal to Cyclophilin A
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cat, Pig, Monkey
Synthetic peptide corresponding to a region within amino acids 93-165 of Human Cyclophilin A (Uniprot P62937).
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; methanol-fixed HeLa cells; Human MDA-MB-468 xenograft tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 18 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or Tris-EDTA buffer pH8.0|
FunctionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Sequence similaritiesBelongs to the cyclophilin-type PPIase family. PPIase A subfamily.
Contains 1 PPIase cyclophilin-type domain.
- Information by UniProt
- Cyclophilin A antibody
- Cyclophilin antibody
- CyclophilinA antibody
All lanes : Anti-Cyclophilin A antibody (ab137684) at 1/5000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 18 kDa
12% SDS PAGE
Immunofluorescence analysis of methanol-fixed HeLa cells labelling Cyclophillin A with ab137684 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human MDA-MB-468 xenograft tissue labelling Cyclophillin A with ab137684 at 1/500 dilution.
ab137684 has not yet been referenced specifically in any publications.