Overview

  • Product name

    Anti-Cyclophilin A antibody
    See all Cyclophilin A primary antibodies
  • Description

    Rabbit polyclonal to Cyclophilin A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cat, Pig, Monkey
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 93-165 of Human Cyclophilin A (Uniprot P62937).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; methanol-fixed HeLa cells; Human MDA-MB-468 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or Tris-EDTA buffer pH8.0

Target

Images

  • All lanes : Anti-Cyclophilin A antibody (ab137684) at 1/5000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 18 kDa



    12% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labelling Cyclophillin A with ab137684 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human MDA-MB-468 xenograft tissue labelling Cyclophillin A with ab137684 at 1/500 dilution.

References

ab137684 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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