Anti-Cyclophilin A antibody (ab154388)
Key features and details
- Rabbit polyclonal to Cyclophilin A
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cyclophilin A antibody
See all Cyclophilin A primary antibodies -
Description
Rabbit polyclonal to Cyclophilin A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Pig, Monkey -
Immunogen
Recombinant fragment corresponding to a region within amino acids 1-165 of Human Cyclophilin A (Uniprot ID: P62937)
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Positive control
- 293T, H1299, HeLa, HepG2, Molt-4, Raji and Mouse brain whole cell lysates. SW480 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 18 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 18 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
Contains 1 PPIase cyclophilin-type domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5478 Human
- Entrez Gene: 268373 Mouse
- Entrez Gene: 100360977 Rat
- Entrez Gene: 100362969 Rat
- Entrez Gene: 25518 Rat
- Omim: 123840 Human
- SwissProt: P62937 Human
- SwissProt: P17742 Mouse
see all -
Alternative names
- Cyclophilin A antibody
- Cyclophilin antibody
- CyclophilinA antibody
see all
Images
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All lanes : Anti-Cyclophilin A antibody (ab154388) at 1/1000 dilution
Lane 1 : Molt-4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 18 kDa
12% SDS-PAGE -
Anti-Cyclophilin A antibody (ab154388) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 18 kDa
12% SDS-PAGE -
Immunohistochemical analysis of paraffin-embedded SW480 xenograft labeling Cyclophilin A with ab154388 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154388 has been referenced in 2 publications.
- Lassiter R et al. Protective Role of Kynurenine 3-Monooxygenase in Allograft Rejection and Tubular Injury in Kidney Transplantation. Front Immunol 12:671025 (2021). PubMed: 34305900
- Qi ZY et al. CYPA promotes the progression and metastasis of serous ovarian cancer (SOC) in vitro and in vivo. J Ovarian Res 12:118 (2019). PubMed: 31783885