Other product details
wash 3 PBS+Tween and 3X PBS
cyclophilin (0.3 ug/mL. Affinity Bioreagents PAa1-025) + immunosuppressor (10000 to 0.168 ng/mL) was added and incubated overnight (COMPETITION ofr cyclophilin.
rabbit anti cyclophilin A was added overninght 4C
goat anti rabbit 2 hr 37C prior alkaline phosphatase Sigma (P7998)
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Submitted Jul 31 2006