Overview

  • Product name
    Anti-Cyclophilin A antibody
    See all Cyclophilin A primary antibodies
  • Description
    Rabbit polyclonal to Cyclophilin A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig, Monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Cyclophilin A.

    Read Abcam's proprietary immunogen policy (Peptide available as ab42406.)

  • Positive control
    • Recombinant human Cyclophilin A protein (ab86219) can be used as a positive control in WB. This antibody gave a positive signal in the following human lysates: HeLa (Human epithelial carcinoma cell line) whole cell A431 (Human epithelial carcinoma cell line) whole cell Lymph node (Human) whole cell - adult normal tissue Thymus tissue This antibody gave a positive signal in the following rat lysate: Lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab41684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

Images

  • All lanes : Anti-Cyclophilin A antibody (ab41684) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 4 : Human thymus tissue lysate - total protein (ab30146)
    Lane 5 : Lung (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa

  • ICC/IF image of ab41684 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41684, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • ab41684 staining mouse heart and rat thymus tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C.  The primay antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C.  A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence analysis of HepaRG cells labeling Cyclophilin A with ab41684 at 1μg/ml. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 in PBS. The cells were blocked with 1% milk for 20 minutes at room temperature. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/400 dilution.

    See Abreview

  • ab88202 is a GST-tagged recombinant protein with an expected molecular weight of 50kDa.

References

This product has been referenced in:
  • Li T  et al. Ubiquitin-specific protease 4 promotes hepatocellular carcinoma progression via cyclophilin A stabilization and deubiquitination. Cell Death Dis 9:148 (2018). Read more (PubMed: 29396555) »
  • Antoniou A  et al. The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development. EMBO Rep 19:N/A (2018). WB ; Rat . Read more (PubMed: 29263199) »
See all 26 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Skin)
Antigen retrieval step
None
Permeabilization
No
Specification
Skin
Blocking step
(agent) for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 24 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepaRG)
Specification
HepaRG
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C

Mrs. Alina Macovei

Verified customer

Submitted May 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepaRG)
Loading amount
10 µg
Specification
HepaRG
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Mrs. Alina Macovei

Verified customer

Submitted May 03 2012

Answer

Thank you for your message and for confirming these details. I am very pleased to hear the customer would like to accept our offer and test these antibodies in hamster. ab41684 Discount code: #### ab38975 Discount code: #### ab6314 Discount code: #### ab77179 Discount code: #### Expirey date for all these codes: 7th March 2012 This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit the Abreviews for hamster samples and include the relevant code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. If you have any further questions, please do not hesitate to contact us. We look forward to receiving the Abreviews and wish the customer luck with their research.

Read More

Answer

Thank you for your message which has been forwarded to me as Karin is currently away from the office. I have reviewed these three antibodies with respect to discount codes: ab77179 I am sorry I am also unable to check the alignment of the immunogen with the hamster sequence, because there is regrettably no hamster sequence available on the protein database sites. I would be pleased to make an exception and provide a discount code in this case anyway, to test in Hamster. ab6314 There is 73% Alignment of the human full length immunogen to the hamster sequence you have provided. This is a little low, but there may be some crossreactivity so I would be pleased to provide a discount. ab16045 There is 14% alignment of the hamster sequence provided with the immunogen. This is very low so the antibody will probably not detect in hamster, and so I am sorry we would be unable to provide a discount for this particular antibody to test in hamster. I hope this will be helpful to you. Please confirm if the customer would like to receive a code for testing ab6314 and ab77179 in hamster and I will be pleased to arrange this for you.

Read More

Answer

Thank you for contacting us. If an antibody has not been tested with a particular species then a testing discount would apply. However, in order to check the likelihood of the antibody being reactive against the target the hamster protein sequence needs to be compared to that of the immunogen used to make sure that the most suitable antibody is chosen. Unfortunately I have been unable to find sequences for Entactin, Cathepsin L or Cyclophilin B for hamster so if you or your customer could help me with this that would be very useful. We already have several antibodies directed against Hsp70 which have been tested with hamster samples. ab47454 is a mouse monoclonal or ab79852 is a rabbit polyclonal. We also have several which are directly conjugated that may be of interest too. These can be found from the following link: https://www.abcam.com/index.html?pageconfig=searchresults&search=hsp70&pt=1&sk=app&sv=69&sn=WB&l=3&fViewMore=1 The Cyclophilin A antibody suggested (ab41684) is predicted to react and would therefore be suitable for a testing discount. However, with the TIMP2 antibody suggested (ab109708), the immunogen recognises the N-terminal sequence of the protein which differs significantly from that of the hamster protein. I would therefore recommend ab38975 as an alternative. This is a rabbit polyclonal directed against the C-terminal end which shares significant homology with Chinese hamster sequence (SwissProt Q60453) compared to the human (SwissProt P16035). I hope this information is helpful to you. If you'd like me to issue testing discounts for ab41684 or ab38975. Or you would like to stick with the original Hsp70 antibody recommended please let me know. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
20 µg
Specification
293T
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dr. Pankaj Gupta

Verified customer

Submitted Sep 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse (heart), rat (thymus))
Specification
mouse (heart), rat (thymus)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Sequential peroxidase and protein block (prediluted) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Apr 03 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF, NIH-3T3, RAW)
Loading amount
20 µg
Specification
MEF, NIH-3T3, RAW
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

Submitted Apr 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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