Anti-Cyclophilin A antibody (ab41684)
Key features and details
- Rabbit polyclonal to Cyclophilin A
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Cyclophilin A antibody
See all Cyclophilin A primary antibodies -
Description
Rabbit polyclonal to Cyclophilin A -
Host species
Rabbit -
Specificity
Replenishment batches of our polyclonal antibody, ab41684 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab126738.
-
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Dog, Pig, Monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Cyclophilin A.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
|
ICC/IF | (1) |
Use a concentration of 1 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
-
Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
Contains 1 PPIase cyclophilin-type domain. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 5478 Human
- Entrez Gene: 268373 Mouse
- Entrez Gene: 100360977 Rat
- Entrez Gene: 100362969 Rat
- Entrez Gene: 25518 Rat
- Omim: 123840 Human
- SwissProt: P62937 Human
- SwissProt: P17742 Mouse
see all -
Alternative names
- Cyclophilin A antibody
- Cyclophilin antibody
- CyclophilinA antibody
see all
Images
-
All lanes : Anti-Cyclophilin A antibody (ab41684) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Human thymus tissue lysate - total protein (ab30146)
Lane 5 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa -
ICC/IF image of ab41684 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41684, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
-
Immunocytochemistry/ Immunofluorescence analysis of HepaRG cells labeling Cyclophilin A with ab41684 at 1μg/ml. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 in PBS. The cells were blocked with 1% milk for 20 minutes at room temperature. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/400 dilution.
-
ab88202 is a GST-tagged recombinant protein with an expected molecular weight of 50kDa.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (53)
ab41684 has been referenced in 53 publications.
- Yilmaz DE et al. Immunosuppressive calcineurin inhibitor cyclosporine A induces proapoptotic endoplasmic reticulum stress in renal tubular cells. J Biol Chem 298:101589 (2022). PubMed: 35033536
- Grunwald SA et al. Statins Aggravate the Risk of Insulin Resistance in Human Muscle. Int J Mol Sci 23:N/A (2022). PubMed: 35216514
- Li L et al. miR-15a and miR-20b sensitize hepatocellular carcinoma cells to sorafenib through repressing CDC37L1 and consequent PPIA downregulation. Cell Death Discov 8:297 (2022). PubMed: 35760798
- Pang J et al. Association of Pericyte Loss With Microthrombosis After Subarachnoid Hemorrhage in ApoE-Deficient Mice. Front Neurol 12:726520 (2021). PubMed: 34566870
- Anandan V et al. Cyclophilin A Impairs Efferocytosis and Accelerates Atherosclerosis by Overexpressing CD 47 and Down-Regulating Calreticulin. Cells 10:N/A (2021). PubMed: 34944106