Product nameAnti-Cyclophilin A antibody
See all Cyclophilin A primary antibodies
DescriptionRabbit polyclonal to Cyclophilin A
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig, Monkey, Gorilla, Chinese hamster, Orangutan
- Recombinant human Cyclophilin A protein (ab86219) can be used as a positive control in WB. This antibody gave a positive signal in the following human lysates: HeLa (Human epithelial carcinoma cell line) whole cell A431 (Human epithelial carcinoma cell line) whole cell Lymph node (Human) whole cell - adult normal tissue Thymus tissue This antibody gave a positive signal in the following rat lysate: Lung tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab41684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Sequence similaritiesBelongs to the cyclophilin-type PPIase family. PPIase A subfamily.
Contains 1 PPIase cyclophilin-type domain.
- Information by UniProt
- Cyclophilin A antibody
- Cyclophilin antibody
- CyclophilinA antibody
All lanes : Anti-Cyclophilin A antibody (ab41684) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Human thymus tissue lysate - total protein (ab30146)
Lane 5 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
ICC/IF image of ab41684 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41684, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab41684 staining mouse heart and rat thymus tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C. The primay antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.
Immunocytochemistry/ Immunofluorescence analysis of HepaRG cells labeling Cyclophilin A with ab41684 at 1μg/ml. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 in PBS. The cells were blocked with 1% milk for 20 minutes at room temperature. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/400 dilution.
ab88202 is a GST-tagged recombinant protein with an expected molecular weight of 50kDa.
This product has been referenced in:
- Rodrigues IS et al. Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida. Sci Rep 9:9019 (2019). Read more (PubMed: 31227743) »
- Li T et al. Ubiquitin-specific protease 4 promotes hepatocellular carcinoma progression via cyclophilin A stabilization and deubiquitination. Cell Death Dis 9:148 (2018). Read more (PubMed: 29396555) »