For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Dear Tech support, I have a researcher who is looking to test a number of antibodies against hamster tissue for western blot. Below are the antibodies of interest. As these antibodies have not been validated against hamster would a testing discount apply in this case? ab77179 Anti-Entactin ab5439 Anti-Hsp70 ab6314 Anti-Cathepsin L ab16045 Anti-Cyclophilin B ab109708 Anti-TIMP2 ab41684 Anti-Cyclophilin A Could you please let me know if you could offer the testing discount or if there are any guarantees for the antibodies as hamster is not listed in the validated species. Hope to hear from you soon. Best regards,
Asked on Oct 20 2011
Thank you for contacting us. If an antibody has not been tested with a particular species then a testing discount would apply. However, in order to check the likelihood of the antibody being reactive against the target the hamster protein sequence needs to be compared to that of the immunogen used to make sure that the most suitable antibody is chosen. Unfortunately I have been unable to find sequences for Entactin, Cathepsin L or Cyclophilin B for hamster so if you or your customer could help me with this that would be very useful. We already have several antibodies directed against Hsp70 which have been tested with hamster samples. ab47454 is a mouse monoclonal or ab79852 is a rabbit polyclonal. We also have several which are directly conjugated that may be of interest too. These can be found from the following link: https://www.abcam.com/index.html?pageconfig=searchresults&search=hsp70&pt=1&sk=app&sv=69&sn=WB&l=3&fViewMore=1 The Cyclophilin A antibody suggested (ab41684) is predicted to react and would therefore be suitable for a testing discount. However, with the TIMP2 antibody suggested (ab109708), the immunogen recognises the N-terminal sequence of the protein which differs significantly from that of the hamster protein. I would therefore recommend ab38975 as an alternative. This is a rabbit polyclonal directed against the C-terminal end which shares significant homology with Chinese hamster sequence (SwissProt Q60453) compared to the human (SwissProt P16035). I hope this information is helpful to you. If you'd like me to issue testing discounts for ab41684 or ab38975. Or you would like to stick with the original Hsp70 antibody recommended please let me know. Please do not hesitate to contact us if you need any more advice or information.
Answered on Oct 20 2011