Western blot abreview for Anti-Cyclophilin A antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF, NIH-3T3, RAW)
Loading amount
20 µg
Specification
MEF, NIH-3T3, RAW
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% Milk PBS-Tween 20 (0.1%)

Secondary antibody

Name
Non-Abcam antibody was used: Swine anti Rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
Supersignal West Dura
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 18 kDa Non-specific: 48 (faint) kDa

Additional data

Additional Notes
Lane order: MEF (lane 1), NIH-3T3 (lane 2), RAW (lane 3).
Detects a species of the predicted mobility (18 kDa) in all lysates tested. A faint cross-reactive band observed at 48 kDa.

Mr. Antibody Solutions

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Submitted Apr 01 2008

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