Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cyclophilin A antibody

Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Skin)
Antigen retrieval step
None
Permeabilization
No
Specification
Skin
Blocking step
(agent) for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Biogenex Common Antibody Diluent
Dilution
1/20

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-rabbit IgG AF488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
The staining with this antibody was good, but the concentration required was very high, which is why it was not given a higher rating.

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Submitted Dec 24 2015

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