• Product name

    Anti-Cyclophilin A antibody
    See all Cyclophilin A primary antibodies
  • Description

    Mouse monoclonal to Cyclophilin A
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IF, ELISA, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Cyclophilin A aa 1-165.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.



Our Abpromise guarantee covers the use of ab58144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration. It can be applied in indirect ELISA (at a dilution of 1/1500 for 24 hrs).
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.



  • Function

    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Sequence similarities

    Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
    Contains 1 PPIase cyclophilin-type domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Cyclophilin A antibody
    • Cyclophilin antibody
    • CyclophilinA antibody
    • Cyclosporin A binding protein antibody
    • Cyclosporin A-binding protein antibody
    • CYPA antibody
    • CYPH antibody
    • Epididymis secretory sperm binding protein Li 69p antibody
    • HEL S 69p antibody
    • MGC117158 antibody
    • MGC12404 antibody
    • MGC23397 antibody
    • Peptidyl prolyl cis trans isomerase A antibody
    • Peptidyl-prolyl cis-trans isomerase A antibody
    • Peptidylprolyl isomerase A (cyclophilin A) antibody
    • Peptidylprolyl isomerase A antibody
    • PPIA antibody
    • PPIA protein antibody
    • PPIA_HUMAN antibody
    • PPIase A antibody
    • Rotamase A antibody
    • RotamaseA antibody
    • T cell cyclophilin antibody
    see all


  • Cyclophilin A antibody (ab58144) at 1ug/lane + Jurkat cell lysate at 25ug/lane.

    This image was generated using the ascites version of the product.

  • ICC/IF image of ab58144 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58144, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This image was generated using the ascites version of the product.

  • Cyclophilin A was immunoprecipitated using 0.5mg Hela whole cell extract, 10µg of Mouse monoclonal to Cyclophilin A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab58144.
    Secondary: Protein G-HRP at 1/500 dilution.
    Band: 18kDa: Cyclophilin A.

    This image was generated using the ascites version of the product.

  • Overlay histogram showing HeLa cells stained with ab58144 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58144, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

  • This image shows an ELISA analysis of ab58144. 

    PBMC huh-7 cells were incubated with medium + 10% FCS.  At the end of the incubation the supernatant was collected and cells washed with cold PBS.  Cells were scrapped and lysate generated.  The cell lysate and extracellular medium were assyed for Cyclphilin A content in a sandwich ELISA.  The primary antibody was diluted 1/1500 and incubated with the sample for 24 hours at 4°C.  Ab6729 was diluted 1/2000.

    This image was generated using the ascites version of the product.

    Key for lower diagram:

    1) Rabbit anti-Cyclophilin A (ab3563)

    2) Cells samples or Std (ab56523)

    3) Mouse anti-Cyclophilin A (ab58144)

    4) Rabbit anti-mouse Alkaline Phosphatase (ab6729)

    5) Substrate of Alkaline Phosphatase

    Standards = Recombinant protein Cyclophilin A (ab56523)

    See Abreview


This product has been referenced in:

  • Zhang L  et al. H19 potentiates let-7 family expression through reducing PTBP1 binding to their precursors in cholestasis. Cell Death Dis 10:168 (2019). Read more (PubMed: 30778047) »
  • Jiao Y  et al. Discovering metabolic disease gene interactions by correlated effects on cellular morphology. Mol Metab 24:108-119 (2019). Read more (PubMed: 30940487) »
See all 13 Publications for this product

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1-2 of 2 Abreviews

Western blot
Human Tissue lysate - whole (endometrial cancer and adjacent control endometriu)
Loading amount
60 µg
endometrial cancer and adjacent control endometriu
Gel Running Conditions
Reduced Denaturing (10 % Tris-Glicine gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 07 2011

Human Cell (PBMC huh-7 cells)
PBMC huh-7 cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Sandwich (Detection)

Abcam user community

Verified customer

Submitted Jun 30 2008

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