ELISA abreview for Anti-Cyclophilin A antibody

Excellent
Abreviews
Application
ELISA
Sample
Human Cell (PBMC huh-7 cells)
Specification
PBMC huh-7 cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Type
Sandwich (Detection)

Other product details

Dilution
1/1500
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS pH 7.4

Secondary antibody

Dilution
1/2000

Additional data

Additional Notes
cells [PBMC or huh-7] incubated with medium + 10 % FCS at the end of the incubation supernatant was collected and cells washed with cold PBS.
cells were scrapped and lysate generated
cell lysate and extracellular medium were assayed for Cyclophilin A content

Abcam user community

Verified customer

Submitted Jun 30 2008

Sign up