Western blot abreview for Anti-Cyclophilin B antibody

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Kidney)
Loading amount
20 µg
Specification
Kidney
Gel Running Conditions
Reduced Denaturing (10% Bis Tris Gels)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1250

Detection

Detection method
ECL
Exposure
15 second(s)
Bands
Specific: 21 kDa
Negative control
No primary negative and isotype control - Both clear

Additional data

Additional Notes
Lane 1 = Rat Kidney 20µg
Lane 2 = Rat Kidney 30µg

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Submitted Mar 19 2010

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