Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclophilin B antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (panceatic AR42J cells)
Specification
panceatic AR42J cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C

Other product details

Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat-anti-Rabbit-TRITC
Host species: Goat
Clonality: Polyclonal
Conjugation: TRITC
Dilution
1/100

Additional data

PubMed ID
20707389: View
Additional Notes
4% pFA for 20 min 0.2% Tx-100 for 10 min, all in PBS
Cyclophilin B colocalizes with the ER marker GFP-Sec61beta in pancreatic AR42J cells

Dr. Michael Schrader

Verified customer

Submitted Sep 14 2010

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